Abstract
Methyl ethyl ketone (MEK) is a common groundwater contaminant often present with more toxic compounds of primary interest. Because of this, few studies have been performed to determine the effect of microbial community structure on MEK biodegradation rates in aquifer sediments. Here, microcosms were prepared with aquifer sediments containing MEK following a massive spill event and compared to laboratory-spiked sediments, with MEK biodegradation rates quantified under mixed aerobic/anaerobic conditions. Biodegradation was achieved in MEK-contaminated site sediment microcosms at about half of the solubility (356 mg/L) with largely Firmicutes population under iron-reducing conditions. MEK was biodegraded at a higher rate [4.0 ± 0.74 mg/(L days)] in previously exposed site samples compared to previously uncontaminated sediments [0.51 ± 0.14 mg/(L days)]. Amplicon sequencing and denaturing gradient gel electrophoresis of 16S rRNA genes were combined to understand the relationship between contamination levels, biodegradation, and community structure across the plume. More heavily contaminated sediments collected from an MEK-contaminated field site had the most similar communities than less contaminated sediments from the same site despite differences in sediment texture. The more diverse microbial community observed in the laboratory-spiked sediments reduced MEK concentration 47 % over 92 days. Results of this study suggest lower rates of MEK biodegradation in iron-reducing aquifer sediments than previously reported for methanogenic conditions and biodegradation rates comparable to previously reported nitrate- and sulfate-reducing conditions.
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Acknowledgments
The authors wish to thank Jody Smiley (Virginia Tech) for chemistry analytical assistance, Marian Alicea (Virginia Tech), Pan Ji (Virginia Tech), and Hannah Delos Reyes (Rutgers University) for assistance preparing samples for amplicon sequencing. We thank the Atlanta office of AMEC Foster Wheeler for their assistance with sample collection and site data.
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Fahrenfeld, N., Pruden, A. & Widdowson, M. Kinetic and microbial community analysis of methyl ethyl ketone biodegradation in aquifer sediments. Biodegradation 28, 27–36 (2017). https://doi.org/10.1007/s10532-016-9775-4
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DOI: https://doi.org/10.1007/s10532-016-9775-4