Abstract
The bioaugmentation strains for phenol, pyridine, quinoline, carbazole, and naphthalene degradation were employed to treat coking wastewater in a membrane bioreactor (MBR). The results showed that the bioaugmented MBR was much better in pollutant removal than that of the control MBR with conventional activated sludge. Compared to the control MBR, the bioaugmented MBR displayed an additional 3.2 mg/L of phenol, pyridine, quinoline, naphthalene and carbazole in total by the addition of the degrading strains. Also, about 10 % of the chemical oxygen demand in the effluent was further removed by the bioaugmentation. The pyrosequencing analysis of the sludge in the MBRs revealed that the microbial community shifted in response to the addition of the degrading strains. The diversity of the microbial community increased during the bioaugmentation, and some bacterial taxa favorable to the removal of toxic and refractory pollutants appeared in the bioaugmented MBR. The results indicated that the use of high-efficiency bacteria was a feasible method for industrial coking wastewater treatment.
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References
APHA (2005) Standard methods for the examination of water and wastewater, 21st edn. American Public Health Association, Washington, DC
Aslam Z, Im WT, Kim MK, Lee ST (2005) Flavobacterium granuli sp. nov., isolated from granules used in a wastewater treatment plant. Int J Syst Evol Microbiol 55:747–751
Bai Y, Sun Q, Zhao C, Wen D, Tang X (2008) Microbial degradation and metabolic pathway of pyridine by a Paracoccus sp. strain BW001. Biodegradation 19:915–926
Bai YH, Sun QH, Zhao C, Wen DH, Tang XY (2010) Bioaugmentation treatment for coking wastewater containing pyridine and quinoline in a sequencing batch reactor. Appl Microbiol Biotechnol 87:1943–1951
Bai Y, Sun Q, Sun R, Wen D, Tang X (2011) Bioaugmentation and adsorption treatment of coking wastewater containing pyridine and quinoline using zeolite-biological aerated filters. Environ Sci Technol 45:1940–1948
Bai Y, Shi Q, Wen D, Li Z, Jefferson WA, Feng C, Tang X (2012) Bacterial communities in the sediments of Dianchi Lake, a partitioned eutrophic waterbody in China. PLoS ONE 7:e37796
Boon N, Top EM, Verstraete W, Siciliano SD (2003) Bioaugmentation as a tool to protect the structure and function of an activated-sludge microbial community against a 3-chloroaniline shock load. Appl Environ Microbiol 69:1511–1520
Fuller ME et al (2014) Laboratory evaluation of bioaugmentation for aerobic treatment of RDX in groundwater. Biodegradation 26:77–89
Huson DH, Auch AF, Qi J, Schuster SC (2007) MEGAN analysis of metagenomic data. Genome Res 17:377–386
Ikuma K, Gunsch CK (2012) Genetic bioaugmentation as an effective method for in situ bioremediation: functionality of catabolic plasmids following conjugal transfers. Bioengineered 3:236–241
Jones SE, Lennon JT (2010) Dormancy contributes to the maintenance of microbial diversity. Proc Natl Acad Sci USA 107:5881–5886
Kim YM, Park D, Lee DS, Park JM (2008) Inhibitory effects of toxic compounds on nitrification process for cokes wastewater treatment. J Hazard Mater 152:915–921
Kim YM, Cho HU, Lee DS, Park C, Park D, Park JM (2011) Response of nitrifying bacterial communities to the increased thiocyanate concentration in pre-denitrification process. Bioresour Technol 102:913–922
Kraigher B, Kosjek T, Heath E, Kompare B, Mandic-Mulec I (2008) Influence of pharmaceutical residues on the structure of activated sludge bacterial communities in wastewater treatment bioreactors. Water Res 42:4578–4588
Lee TK, Doan TV, Yoo K, Choi S, Kim C, Park J (2010) Discovery of commonly existing anode biofilm microbes in two different wastewater treatment MFCs using FLX titanium pyrosequencing. Appl Microbiol Biotechnol 87:2335–2343
Lu Y, Yan LH, Wang Y, Zhou SF, Fu JJ, Zhang JF (2009) Biodegradation of phenolic compounds from coking wastewater by immobilized white rot fungus Phanerochaete chrysosporium. J Hazard Mater 165:1091–1097
Manefield M, Griffiths RI, Leigh MB, Fisher R, Whiteley AS (2005) Functional and compositional comparison of two activated sludge communities remediating coking effluent. Environ Microbiol 7:715–722
Park S, Yu J, Byun I, Cho S, Park T, Lee T (2011) Microbial community structure and dynamics in a mixotrophic nitrogen removal process using recycled spent caustic under different loading conditions. Bioresour Technol 102:7265–7271
Paul L, Herrmann S, Koch CB, Philips J, Smolders E (2013) Inhibition of microbial trichloroethylene dechorination by Fe(III) reduction depends on Fe mineralogy: a batch study using the bioaugmentation culture KB-1. Water Res 47:2543–2554
Puyol D, Monsalvo VM, Sanchis S, Sanz JL, Mohedano AF, Rodriguez JJ (2015) Comparison of bioaugmented EGSB and GAC-FBB reactors and their combination with aerobic SBR for the abatement of chlorophenols. Chem Eng J 259:277–285
Scheutz C, Durant ND, Broholm MM (2014) Effects of bioaugmentation on enhanced reductive dechlorination of 1,1,1-trichloroethane in groundwater: a comparison of three sites. Biodegradation 25:459–478
Schloss PD et al (2009) Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 75:7537–7541
Shokrollahzadeh S, Azizmohseni F, Golmohammad F, Shokouhi H, Khademhaghighat F (2008) Biodegradation potential and bacterial diversity of a petrochemical wastewater treatment plant in Iran. Bioresour Technol 99:6127–6133
Wagner M, Loy A (2002) Bacterial community composition and function in sewage treatment systems. Curr Opin Biotechnol 13:218–227
Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
Yin N, Yang G, Zhong ZX, Xing WH (2011) Separation of ammonium salts from coking wastewater with nanofiltration combined with diafiltration. Desalination 268:233–237
Zhao C, Zhang Y, Li X, Wen D, Tang X (2011) Biodegradation of carbazole by the seven Pseudomonas sp. strains and their denitrification potential. J Hazard Mater 190:253–259
Zhou NA, Lutovsky AC, Andaker GL, Gough HL, Ferguson JF (2013) Cultivation and characterization of bacterial isolates capable of degrading pharmaceutical and personal care products for improved removal in activated sludge wastewater treatment. Biodegradation 24:813–827
Zhu X, Tian J, Chen L (2012) Phenol degradation by isolated bacterial strains: kinetics study and application in coking wastewater treatment. J Chem Technol Biotechnol 87:123–129
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This study was supported by the National Natural Science Foundation of China (No. 51308319).
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Zhu, X., Liu, R., Liu, C. et al. Bioaugmentation with isolated strains for the removal of toxic and refractory organics from coking wastewater in a membrane bioreactor. Biodegradation 26, 465–474 (2015). https://doi.org/10.1007/s10532-015-9748-z
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DOI: https://doi.org/10.1007/s10532-015-9748-z