Abstract
Objectives
To improve rhamnolipid production and its potential application in removal of crude oil, the recombinant Pseudomonas aeruginosa strain DAB was constructed to enhance yield of rhamnolipids.
Results
Strain DAB had a higher yield of 17.3 g rhamnolipids l−1 in the removal process with crude oil as the sole carbon source than 10 g rhamnolipids l−1 of wild-type strain DN1, where 1% crude oil was degraded more than 95% after 14 days cultivation. These rhamnolipids reduced the surface tension of water from 72.92 to 26.15 mN m−1 with CMC of 90 mg l−1. The predominant rhamnolipid congeners were Rha–C10–C10 and Rha–Rha–C10–C10 detected by MALDI-TOF MS analysis with approx. 70% relative abundance, although a total of 21 rhamnolipid congeners were accumulated.
Conclusion
Increasing the copy number of rhlAB genes efficiently enhanced the production of rhamnolipids by the recombinant P. aeruginosa DAB and thus presents a promising application for the bioremediation process.
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Acknowledgements
This research was supported by the National Science Foundation for Young Scientists of China (Grant No. 31000069) and the research project of Shaanxi Provincial Key Laboratory of Biotechnology (16JS108).
Supplementary information
Supplementary Figure 1—Schematic diagram of the construction of recombinant plasmid.
Supplementary Figure 2—Time course of pollutant degradation by the engineered strain DAB and DN1 after inoculation in the optimized medium consisting of BPLM with different PAHs as the sole carbon source (A) naphathalene; (B) phenanthrene; (C) pyrene; (D) fluoranthene.
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He, C., Dong, W., Li, J. et al. Characterization of rhamnolipid biosurfactants produced by recombinant Pseudomonas aeruginosa strain DAB with removal of crude oil. Biotechnol Lett 39, 1381–1388 (2017). https://doi.org/10.1007/s10529-017-2370-x
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DOI: https://doi.org/10.1007/s10529-017-2370-x