Abstract
Objectives
With the view of designing a single biocatalyst for biorefining, carbazole dioxygenase was cloned from Pseudomonas sp. and expressed in Rhodococcus sp.
Results
The recombinant, IGTS8, degraded both carbazole and dibenzothiophene at 400 mg/l in 24 h. Maximum carbazole degradation was in 1:1 (v/v) hexadecane/aqueous phase. Anthracene, phenanthrene, pyrene, fluoranthene and fluorine were also degraded without affecting the aliphatic component.
Conclusions
Recombinant Rhodococcus sp. IGTS8 can function as a single biocatalyst for removing major contaminants of fossil fuels viz. dibenzothiophene, carbazole and polyaromatic compounds.
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Acknowledgements
We are thankful to Benjamin Stark for providing pRESX. We would also like to thank the Department of Science and Technology, Government of India, for their generous financial support (Project No. SB/YS/LS-223/2013) and Jaypee Institute of Information Technology for providing research fellowship (SK).
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Khan, S., Adhikari, D.K., Gupta, S. et al. Degradation of carbazole, dibenzothiophene and polyaromatic hydrocarbons by recombinant Rhodococcus sp.. Biotechnol Lett 39, 277–281 (2017). https://doi.org/10.1007/s10529-016-2242-9
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DOI: https://doi.org/10.1007/s10529-016-2242-9