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Adsorption induced denaturation: application to denaturation of soybean trypsin inhibitor (SBTI) and lipoxygenase (LOX) in soymilk

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Abstract

Allyl glycidyl ether-ethylene glycol dimethacrylate copolymer with 25 % crosslink density has been functionalized with various amines. The polymer bearing a dibenzyl amino function efficiently removed the soybean trypsin inhibitor (SBTI) and lipoxygenase (LOX) from soymilk. The polymer binds SBTI and LOX efficiently (25–30 mg/g) through a combination of hydrophobic and ionic interactions and denatures them at room temperature by simple contact for 1 h. The bound proteins can be easily recovered almost quantitatively by elution with 1 % (v/v) acetic acid, and the polymer can be recycled at least 10 times without significant changes in binding capacity.

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Acknowledgments

We thank CSIR, New Delhi for grant of Senior Research Fellowship to LSJ and DST, New Delhi for financial support (Grant No. SERB/MOFPI/0049/2012).

Supporting information

Supplementary Figure 1—SDS PAGE Electrophoresis.

Supplementary Figure 2—Recycling of polymer after protein desorption.

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Authors and Affiliations

  1. Natural Products Chemistry Division, Indian Institute of Chemical Technology, Uppal Road, Habsiguda, Hyderabad, 500007, India

    Lakshmi Swarnalatha Jasti, Kuna Lavanya & Nitin W. Fadnavis

Authors
  1. Lakshmi Swarnalatha Jasti
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  2. Kuna Lavanya
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  3. Nitin W. Fadnavis
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Corresponding author

Correspondence to Nitin W. Fadnavis.

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Jasti, L.S., Lavanya, K. & Fadnavis, N.W. Adsorption induced denaturation: application to denaturation of soybean trypsin inhibitor (SBTI) and lipoxygenase (LOX) in soymilk. Biotechnol Lett 37, 147–151 (2015). https://doi.org/10.1007/s10529-014-1659-2

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  • DOI: https://doi.org/10.1007/s10529-014-1659-2

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