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Secretion of biologically active human interleukin 22 (IL-22) by Lactococcus lactis

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Abstract

Interleukin-22 (IL-22) participates in the modulation of innate immunity and inflammation. This cytokine has important therapeutic potential, such as with ulcerative colitis, liver and lung injury, and infection, in different animal models. We generated a Lactococcus lactis strain that secretes human IL-22 under the regulation of the nisin-inducible promoter. Identification and secretion of this cytokine was demonstrated using western blots of culture supernatants from IL-22-expressing bacteria. The recombinant IL-22 protein produced by L. lactis was biologically active as determined by its ability to induce IL-10 secretion when co-cultured with a colon epithelial cell line in vitro. We consider this novel strain a promising live vaccine for various therapeutic applications.

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Acknowledgments

This manuscript was supported by Programa de Apoyo a la Investigación en Ciencia y Tecnología (PAICYT) from the UANL.

Conflict of interest

The authors declare that they have no conflict of interest.

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Author notes

  1. Julio Villatoro-Hernández

    Present address: Health and Nutrition Division, Chr-Hansen, Bøge Alle 10, 2970, Hørsholm, Denmark

Authors and Affiliations

  1. Departamento de Histología, Facultad de Medicina, Universidad Autónoma de Nuevo León (UANL), Madero y Aguirre Pequeño s/n Mitras Centro, 66460, Monterrey, Nuevo León, Mexico

    María J. Loera-Arias, Julio Villatoro-Hernández, Miguel A. Parga-Castillo, Alejandro Salcido-Montenegro, Roberto Montes-de-Oca-Luna & Odila Saucedo-Cárdenas

  2. Servicio de Anatomía Patológica y Citopatología, Hospital Universitario Dr José Eleuterio González de la Facultad de Medicina, UANL, Monterrey, N.L., Mexico

    Oralia Barboza-Quintana

  3. Servicio de Cirugía General del Hospital Universitario Dr José Eleuterio González de la Facultad de Medicina, UANL, Monterrey, N.L., Mexico

    Gerardo E. Muñoz-Maldonado

  4. División de Genética, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social (IMSS), Monterrey, N.L., Mexico

    Odila Saucedo-Cárdenas

Authors
  1. María J. Loera-Arias
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  2. Julio Villatoro-Hernández
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  3. Miguel A. Parga-Castillo
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  4. Alejandro Salcido-Montenegro
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  5. Oralia Barboza-Quintana
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  6. Gerardo E. Muñoz-Maldonado
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  7. Roberto Montes-de-Oca-Luna
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  8. Odila Saucedo-Cárdenas
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Corresponding author

Correspondence to Odila Saucedo-Cárdenas.

Additional information

M. J. Loera-Arias and J. Villatoro-Hernández have contributed equally to this work.

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Loera-Arias, M.J., Villatoro-Hernández, J., Parga-Castillo, M.A. et al. Secretion of biologically active human interleukin 22 (IL-22) by Lactococcus lactis . Biotechnol Lett 36, 2489–2494 (2014). https://doi.org/10.1007/s10529-014-1626-y

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  • DOI: https://doi.org/10.1007/s10529-014-1626-y

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