Abstract
Actinomycetes are remarkable natural sources of active natural molecules and enzymes of considerable industrial value. Streptomyces mobaraensis is the first microorganism found to produce transglutaminase with broad industrial applications. Although transglutaminase in S. mobaraensis has been well studied over the past three decades, the genome of S. mobaraensis and its secondary metabolic potential were poorly reported. Here, we presented the complete genome of S. mobaraensis DSM40587 obtained from the German Collection of Microorganisms and Cell Cultures GmbH. It contains a linear chromosome of 7,633,041 bp and a circular plasmid of 23,857 bp. The chromosome with an average GC content of 73.49% was predicted to harbour 6683 protein-coding genes, seven rRNA and 69 tRNA genes. Comparative genomic analysis reveals its meaningful genomic characterisation. A comprehensive bioinformatics investigation identifies 35 putative BGCs (biosynthesis gene clusters) involved in synthesising various secondary metabolites. Of these, 13 clusters showed high similarity (> 55%) to known BGCs coding for polyketides, nonribosomal peptides, hopene, RiPP (Ribosomally synthesized and post-translationally modified peptides), and others. Furthermore, these BGCs with over 65% similarity to the known BGCs were analysed in detail. The complete genome of S. mobaraensis DSM40587 reveals its capacity to yield diverse bioactive natural products and provides additional insights into discovering novel secondary metabolites.
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Abbreviations
- BGC:
-
Biosynthetic gene cluster
- RiPP:
-
Ribosomally synthesized and post-translationally modified peptide
- NRPS:
-
Non-ribosomal peptide synthetase
- PKS:
-
Polyketide synthase
- GCF:
-
Gene cluster family
- FPPS:
-
Farnesyl pyrophosphate synthetase
- GFPPS:
-
Geranylfarnesyl diphosphate synthase
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Funding
This work was supported by the National Natural Science Foundation of China (31800031), the China Postdoctoral Science Foundation (2019M653760), Natural Science Basic Research Plan in Shaanxi Province of China (2019JQ-046), Educational Commission of Shaanxi Province (20JY009), the Specialized Scientific Research Fund Projects of Academician Shengyong Zhang (18YSZX004), and the doctoral research funding (Grant No. 17SKY013) from Shangluo University.
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JZQ and XLF designed and performed the experiments, and wrote the manuscript. RQZ, WGD, ZXW and JJX assisted the experiments. JW and JMG revised the manuscript. All the authors discussed the results and commented on the manuscript.
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Feng, Xl., Zhang, Rq., Dong, Wg. et al. The Complete Genomic Sequence of Microbial Transglutaminase Producer, Streptomyces mobaraensis DSM40587. Biochem Genet 62, 1087–1102 (2024). https://doi.org/10.1007/s10528-023-10463-0
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DOI: https://doi.org/10.1007/s10528-023-10463-0