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Characterization of Cellulose Synthase A (CESA) Gene Family in Eudicots

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Abstract

Cellulose synthase A (CESA) is a key enzyme involved in the complex process of plant cell wall biosynthesis, and it remains a productive subject for research. We employed systems biology approaches to explore structural diversity of eudicot CESAs by exon–intron organization, mode of duplication, synteny, and splice site analyses. Using a combined phylogenetics and comparative genomics approach coupled with co-expression networks we reconciled the evolution of cellulose synthase gene family in eudicots and found that the basic forms of CESA proteins are retained in angiosperms. Duplications have played an important role in expansion of CESA gene family members in eudicots. Co-expression networks showed that primary and secondary cell wall modules are duplicated in eudicots. We also identified 230 simple sequence repeat markers in 103 eudicot CESAs. The 13 identified conserved motifs in eudicots will provide a basis for gene identification and functional characterization in other plants. Furthermore, we characterized (in silico) eudicot CESAs against senescence and found that expression levels of CESAs decreased during leaf senescence.

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Acknowledgements

This work was carried out with the support of ‘‘Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01133402)’’ Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Department of Biotechnology, Chonnam National University, Chonnam, 59626, Republic of Korea

    Muhammad Amjad Nawaz, Hafiz Mamoon Rehman, Seung Hwan Yang & Gyuhwa Chung

  2. Center for Soybean Research, State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, SAR, China

    Xiao Lin & Ting-Fung Chan

  3. School of Environmental Science and Engineering, Guangzhou University, Guangzhou, 510275, China

    Muhammad Imtiaz

  4. Department of Plant Pathology, University of Agriculture, Faisalabad, 38040, Pakistan

    Muhammad Amjad Ali

  5. Department of Field Crops, Faculty of Agricultural and Natural Science, Abant Izzet Baysal University, 14280, Bolu, Turkey

    Faheem Shehzad Baloch

  6. US-Pakistan Centre for Advanced Studies in Agriculture and Food Security, University of Agriculture, Faisalabad, 38040, Pakistan

    Rana Muhammad Atif

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  1. Muhammad Amjad Nawaz
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Nawaz, M.A., Lin, X., Chan, TF. et al. Characterization of Cellulose Synthase A (CESA) Gene Family in Eudicots. Biochem Genet 57, 248–272 (2019). https://doi.org/10.1007/s10528-018-9888-z

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