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Characterization of an Acellular Dermal Matrix Obtained by Different Detergent-Enzymatic Methods

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Biomedical Engineering Aims and scope

Acellular dermal matrixes (ADM) for reconstructive surgery were made by treating skin samples from rats and humans with sodium dodecylsulfate (SDS), sodium deoxycholate (DOC), saponin, and DNase. A mixture of SDS and DOC removed cell residues effectively, though the ADM had cytotoxic properties. After treatment with 2% DOC, there was no cytotoxic effect and the resulting material contained 27-202 ng/mg dry weight DNA and from 0.07 to 2.6% fat, depending on the source of the samples.

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Authors and Affiliations

  1. Samara State Medical University, Ministry of Health of the Russian Federation, Samara, Russia

    N. N. Sarbaeva, M. N. Milyakova, V. V. Rossinskaya, O. V. Gribkova & D. A. Dolgushkin

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  1. N. N. Sarbaeva
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  2. M. N. Milyakova
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  3. V. V. Rossinskaya
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  4. O. V. Gribkova
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  5. D. A. Dolgushkin
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Correspondence to N. N. Sarbaeva.

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Translated from Meditsinskaya Tekhnika, Vol. 51, No. 3, May-Jun., 2017, pp. 12-14.

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Sarbaeva, N.N., Milyakova, M.N., Rossinskaya, V.V. et al. Characterization of an Acellular Dermal Matrix Obtained by Different Detergent-Enzymatic Methods. Biomed Eng 51, 166–169 (2017). https://doi.org/10.1007/s10527-017-9707-6

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  • DOI: https://doi.org/10.1007/s10527-017-9707-6

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