Abstract
We have standardized the protocols for the development of acellular dermal matrix from sheep skin. Acellular dermal matrices are composed of extracellular matrix (ECM) and are typically derived by decellularization of native tissues. Sodium deoxycholate (SD), sodium dodecyl sulfate (SDS), Triton X-100, and Tween 20 detergents in combination with trypsin enzyme are used to lyse the cells followed by rinsing to remove the cells. The decellularization was confirmed by histological examination. Treatment with 2% SD and 2% SDS for 24 h along with trypsin resulted in complete acellularity of the dermis. The results are more or less similar with these too detergents. We succeeded in preservation of three-dimensional ultrastructure of ECM which is highly desirable and requires proper decisions regarding the agents and techniques involved in the process.
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Kumar, N. et al. (2022). Tissue Scaffolds Derived from Sheep Skin. In: Kumar, N., Kumar, V., Shrivastava, S., Gangwar, A.K., Saxena, S. (eds) Tissue Scaffolds. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2425-8_5
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DOI: https://doi.org/10.1007/978-1-0716-2425-8_5
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