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Identification and characterization of a novel marine Bacillus cereus VCRC-B540 for mosquito control

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Abstract

A marine bacterium with mosquitocidal effect was isolated from the gut region of the marine red snapper fish (Lutjanus sanguineous). The 16S rRNA gene sequence alignment showed that this isolate belonged to the strain Bacillus cereus VCRC-B540 (NCBI: JN377787). Biochemical studies showed that the strain could be useful in mosquito control. It showed an increasing pattern of toxicity for Culex quinquefasciatus, Anopheles stephensi, and Aedes aegypti, without negative effects for the non-targeted organisms Chironomus riparius, Daphnia cephalata and Notonecta glauca. A qualitative analysis of B. cereus showed that a polypeptide (M.wt: 90 kDa) was associated with the toxicity observed. Consequently, the peptide sequence is identified to be a surface layer protein. A phylogenetic analysis of 16S rDNA gene sequence of B. cereus revealed shared homology with the Bacillus species. Hence, it is concluded that the marine bacterium (B. cereus) can be useful in the biological control of mosquito vectors.

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Acknowledgments

The first author acknowledges the Pondicherry Central University for providing the student fellowship for registering his PhD program. The authors acknowledge Dr. P. Jambulingam, The Director of VCRC, Pondicherry-605006, for permission. They also acknowledge Smt. R. Sundarammal, Sr. Library Information Officer, VCRC, Pondicherry for facilitating library resources.

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Authors and Affiliations

  1. Unit of Microbiology and Immunology, Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar, Puducherry, 605 006, India

    Chinnasamy Mani, Krishnaraj Thirugnanasambantham & Subbiah Poopathi

  2. Department of Ecology and Environmental Sciences, School of Life-Sciences, Pondicherry University, Puducherry-, 605014, India

    Somaiah Sundarapandian

Authors
  1. Chinnasamy Mani
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  2. Krishnaraj Thirugnanasambantham
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  3. Somaiah Sundarapandian
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  4. Subbiah Poopathi
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Correspondence to Subbiah Poopathi.

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Handling Editor: Helen Roy.

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Mani, C., Thirugnanasambantham, K., Sundarapandian, S. et al. Identification and characterization of a novel marine Bacillus cereus VCRC-B540 for mosquito control. BioControl 60, 71–79 (2015). https://doi.org/10.1007/s10526-014-9605-8

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  • DOI: https://doi.org/10.1007/s10526-014-9605-8

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