Abstract
Aging dramatically increases the risk of cardiovascular diseases in human. Animal models are of great value to study cardiac aging, and zebrafish have become a popular model for aging study recently. However, there is limited knowledge about the progression and regulation of cardiac aging in zebrafish. In this study we first validated the effectiveness of a panel of aging-related markers and revealed their spatial-temporal specificity. Using these markers, we discovered that cardiac aging in zebrafish initiated at mid-age around 24 months, followed by a gradual progression marked with increased DNA damage, inflammatory response and reduced mitochondrial function. Furthermore, we showed aging-related expression profile change in zebrafish hearts was similar to that in rat hearts. Overall, our results provide a deeper insight into the cardiac aging process in zebrafish, which will set up foundation for generating novel cardiac aging models suitable for large scale screening of pharmaceutical targets.
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Data availability
Transcriptomic data had been uploaded to GEO database which is publicly accessible.
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Acknowledgements
We thank Haitao Zhou and Lifeng Li for fish care, Kaa Seng Lai, Yuanyuan Peng, Yabo Fang and Shuzhang Liang for technical support and other lab members for in depth discussion.
Funding
This study was supported by National Key R&D Program of China Grant 2018YFA0801004 and NSFC Grant 31571492 to R.Z.
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XS and RZ conceived and designed the project. XS, JM and XL conducted the experiments. YF conducted bioinformatics analysis. XS and CL performed statistical analysis. XS, CL and RZ wrote and revised the manuscript. All authors reviewed the manuscript.
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All experiments were performed according to institutional and national animal welfare guidelines.
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Shao, X., Fu, Y., Ma, J. et al. Functional alterations and transcriptomic changes during zebrafish cardiac aging. Biogerontology 21, 637–652 (2020). https://doi.org/10.1007/s10522-020-09881-z
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DOI: https://doi.org/10.1007/s10522-020-09881-z