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Cellular Model of p21-Induced Senescence

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Oncogene-Induced Senescence

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1534))

Abstract

Cellular senescence is a unique process of normal physiology, from embryonic development to aging, also known for its association with a broad range of pathological conditions. Therefore a reliable model of cellular senescence remains an indispensable tool for the investigation of senescence-associated changes and human disease. Here we describe a model of HT1080 fibrosarcoma cells with an inducible senescence phenotype. These cells are equipped with the lac repressor and exogenous p21 under the control of a lac repressor regulated promoter. The senescent phenotype is induced in these cells by isopropyl-β-d-thiogalactopyranoside (IPTG)-inducible expression of senescence-associated cell cycle inhibitor p21Waf1/Cip1/Sdi1.

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Acknowledgments

This work was supported by NIH grant P20GM109091 (M.S., G.S., E.V.B.) and ACS grant IRG-13-043-01 (E.V.B.).

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Authors and Affiliations

  1. Department of Drug Discovery and Biomedical Sciences, South Carolina College of Pharmacy, University of South Carolina, Columbia, SC, USA

    Michael Shtutman, Gary P. Schools & Eugenia V. Broude Ph.D.

  2. PeptiMed, Inc., Madison, WI, USA

    Bey-Dih Chang

Authors
  1. Michael Shtutman
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  2. Bey-Dih Chang
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  3. Gary P. Schools
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  4. Eugenia V. Broude Ph.D.
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Corresponding author

Correspondence to Eugenia V. Broude Ph.D. .

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Editors and Affiliations

  1. Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, New York, USA

    Mikhail A. Nikiforov

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Shtutman, M., Chang, BD., Schools, G.P., Broude, E.V. (2017). Cellular Model of p21-Induced Senescence. In: Nikiforov, M. (eds) Oncogene-Induced Senescence. Methods in Molecular Biology, vol 1534. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6670-7_3

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  • DOI: https://doi.org/10.1007/978-1-4939-6670-7_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6668-4

  • Online ISBN: 978-1-4939-6670-7

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