We performed a comparative assessment of the immunohistochemical distribution of markers of mitochondrial fission (Drp-1), mitochondrial fusion (Mfn-2), and mitochondrial biogenesis (PGC-1α) in pyramidal neurons of different zones of the hippocampus in mice with intrahippocampal administration of β-amyloid peptide 25-35. The most pronounced changes in the dynamics associated with a decrease in the amount of the fission marker and an increase in the amount of the fusion marker were observed in the CA3 field on day 38 after peptide administration. In the CA1 field, a significant decrease in the marker of mitochondrial biogenesis PGC-1α was found on day 38, which can indicate a decrease in the intensity of mitochondrial biogenesis. Early mitochondrial changes can play an important role in the pathogenesis of all types of memory impairment in Alzheimer’s disease.
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References
Lee I, Park SB. Perirhinal cortical inactivation impairs object-in-place memory and disrupts task-dependent firing in hippocampal CA1, but not in CA3. Front. Neural Circuits. 2013;7:134. doi: https://doi.org/10.3389/fncir.2013.00134
Lamptey RNL, Chaulagain B, Trivedi R, Gothwal A, Layek B, Singh J. A review of the common neurodegenerative disorders: current therapeutic approaches and the potential role of nanotherapeutics. Int. J. Mol. Sci. 2022;23(3):1851. doi: https://doi.org/10.3390/ijms23031851
Reddy PH, Yin X, Manczak M, Kumar S, Pradeepkiran JA, Vijayan M, Reddy AP. Mutant APP and amyloid beta-induced defective autophagy, mitophagy, mitochondrial structural and functional changes and synaptic damage in hippocampal neurons from Alzheimer’s disease. Hum. Mol. Genet. 2018;27(14):2502-2516. doi: https://doi.org/10.1093/hmg/ddy154
Pradeepkiran JA, Reddy PH. Defective mitophagy in Alzheimer’s disease. Ageing Res. Rev. 2020;64:101191. doi: https://doi.org/10.1016/j.arr.2020.101191
DuBoff B, Feany M, Götz J. Why size matters — balancing mitochondrial dynamics in Alzheimer’s disease. Trends Neurosci. 2013;36(6):325-335. doi: https://doi.org/10.1016/j.tins.2013.03.002
Sukhorukov VS, Mudzhiri NM, Voronkova AS, Baranich TI, Glinkina VV, Illarioshkin SN. Mitochondrial disorders in Alzheimer’s disease. Biochemistry (Mosc). 2021;86(6):667-679. doi: https://doi.org/10.1134/S0006297921060055
Tilokani L, Nagashima S, Paupe V, Prudent J. Mitochondrial dynamics: overview of molecular mechanisms. Essays Biochem. 2018. Vol. 62, N 3. P. 341-360. doi: https://doi.org/10.1042/EBC20170104
Nikolaeva NS, Yandulova EYu, Aleksandrova YuR, Starikov AS, Neganova ME. The role of a pathological interaction between β-amyloid and mitochondria in the occurrence and development of Alzheimer’s disease. Acta Naturae. 2022;14(3):19-34. doi: https://doi.org/10.32607/actanaturae.11723
Zhang L, Trushin S, Christensen TA, Bachmeier BV, Gateno B, Schroeder A, Yao J, Itoh K, Sesaki H, Poon WW, Gylys KH, Patterson ER, Parisi JE, Diaz Brinton R, Salisbury JL, Trushina E. Altered brain energetics induces mitochondrial fission arrest in Alzheimer’s disease. Sci. Rep. 2016;6:18725. doi: https://doi.org/10.1038/srep18725
Egorova AV, Voronkov DN, Fedorova EN, Baranich TI, Glinkina VV, Sukhorukov VS. Structural and functional features of mitochondria in neurons and gliocytes of various cerebral regions of laboratory rodents. Klin. Eksper. Morfol. 2023;12(2):5-13. Russian. doi: https://doi.org/10.31088/CEM2023.12.2.5-13
Gauthier-Umaña C, Muñoz-Cabrera J, Valderrama M, Múnera A, Nava-Mesa MO. Acute effects of two different species of amyloid-β on oscillatory activity and synaptic plasticity in the commissural CA3-CA1 circuit of the hippocampus. Neural Plast. 2020;2020:8869526. doi: https://doi.org/10.1155/2020/8869526
Averchuk AS, Ryazanova MV, Baranich TI, Stavrovskaya AV, Rozanova NA, Novikova SV, Salmina AB. The Neurotoxic effect of β-amyloid is accompanied by changes in the mitochondrial dynamics and autophagy in neurons and brain endothelial cells in the experimental model of Alzheimer’s disease. Bull. Exp. Biol. Med. 2023;175(3):315-320. doi: https://doi.org/10.1007/s10517-023-05859-2
Tyumentsev MA, Stefanova NA, Kiseleva EV, Kolosova NG. Mitochondria with morphology characteristic for Alzheimer’s disease patients are found in the brain of OXYS rats. Biochemistry (Mosc). 2018;83(9):1083-1088. doi: https://doi.org/10.1134/S0006297918090109
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 176, No. 10, pp. 530-534, October, 2023
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Baranich, T.I., Skvortsova, K.A., Voronkov, D.N. et al. Immunohistochemical Analysis of Mitochondrial Dynamics in Different Zones of the Hippocampus during Experimental Modeling of Alzheimer’s Disease. Bull Exp Biol Med 176, 523–527 (2024). https://doi.org/10.1007/s10517-024-06060-9
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DOI: https://doi.org/10.1007/s10517-024-06060-9