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Alpha-Fetoprotein as a Factor of Differentiation and Functional Activity of Myeloid-Derived Suppressor Cells

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We studied the role of alpha-fetoprotein (AFP) in regulation of differentiation and functional activity of human myeloid-derived suppressor cells (MDSC) in vitro. To obtain MDSC, CD11b+ cells were isolated from the peripheral blood of healthy donors followed by cytokine induction (IL-1β+GM-CSF) into the MDSC phenotype. The cell functions were assessed by the expression of indoleamine 2,3-dioxygenase (IDO) and arginase-1 (Arg1) and cytokine profile of the cell cultures. Native AFP did not affect the total number of MDSC and the percentage of polymorphonuclear MDSC (PMN-MDSC), but increased the number of monocytic MDSC (M-MDSC). AFP did not change the expression of Arg1, but in low concentrations (10 and 50 U/ml) increased the number of IDO-containing cells. AFP modulated the cytokine profile of CD11b+ cells: it reliably decreased the level of IL-19 (50 and100 U/ml) and showed a tendency to decrease the levels of IL-34, MMP-2, sCD163, CHI3L1, OPN and to increase the levels of IL-29, IL-32, APRIL, PTX3, and sTNF-R1. Thus, we have demonstrated a regulatory effect of native AFP at the level of MDSC generated from CD11b+ cells under conditions of cytokine induction in vitro.

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Authors and Affiliations

  1. Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences — Branch of Perm Federal Research Center, Ural Division of the Russian Academy of Sciences, Perm, Russia

    K. Yu. Shardina, S. A. Zamorina, V. P. Timganova, M. S. Bochkova, S. V. Uzhviyuk & V. A. Chereshnev

  2. Perm State National Research University, Perm, Russia

    S. A. Zamorina, M. S. Bochkova & V. A. Chereshnev

Authors
  1. K. Yu. Shardina
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  2. S. A. Zamorina
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  3. V. P. Timganova
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  4. M. S. Bochkova
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  5. S. V. Uzhviyuk
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  6. V. A. Chereshnev
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Correspondence to K. Yu. Shardina.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 2, pp. 83-91, June, 2023

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Shardina, K.Y., Zamorina, S.A., Timganova, V.P. et al. Alpha-Fetoprotein as a Factor of Differentiation and Functional Activity of Myeloid-Derived Suppressor Cells. Bull Exp Biol Med 175, 535–543 (2023). https://doi.org/10.1007/s10517-023-05901-3

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