Skip to main content

Advertisement

SpringerLink
Log in

Exosomes Derived from Endothelial Cells Inhibit Neointimal Hyperplasia Induced by Carotid Artery Injury in Rats via ROS-NLRP3 Inflammasome Pathway

  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

This study attempted to investigate whether exosomes derived from rat endothelial cells (EC-Exo) attenuate intimal hyperplasia after balloon injury using hematoxylin and eosin staining, immunohistochemistry, immunofluorescence staining, Evans blue staining, and Western blotting. The results indicated that EC-Exo inhibited intimal hyperplasia in the carotid artery after balloon injury, promoted re-endothelialization, and reduced vascular inflammation and ROS-NLRP3-mediated cell pyroptosis. Thus, EC-Exo can inhibit neointimal hyperplasia after carotid artery injury in rats presumably by inhibiting the ROS-NLRP3 inflammasome and phenotypic transformation of vascular smooth muscle cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Siontis GC, Stefanini GG, Mavridis D, Siontis KC, Alfonso F, Pérez-Vizcayno MJ, Byrne RA, Kastrati A, Meier B, Salanti G, Jüni P, Windecker S. Percutaneous coronary interventional strategies for treatment of in-stent restenosis: a network meta-analysis. Lancet. 2015;386:655-664. https://doi.org/10.1016/S0140-6736(15)60657-2

    Article  PubMed  Google Scholar 

  2. Zhu H, Zhang Y. Life and Death Partners in Post-PCI Restenosis: Apoptosis, Autophagy, and The Cross-talk Between Them. Curr Drug Targets. 2018;19(9):1003-1008. https://doi.org/10.2174/1389450117666160625072521

    Article  CAS  PubMed  Google Scholar 

  3. Zuurbier CJ, Abbate A, Cabrera-Fuentes HA, Cohen MV, Collino M, De Kleijn DPV, Downey JM, Pagliaro P, Preissner KT, Takahashi M, Davidson SM. Innate immunity as a target for acute cardioprotection. Cardiovasc. Res. 2019;115(7):1131-1142. https://doi.org/10.1093/cvr/cvy304

    Article  CAS  PubMed  Google Scholar 

  4. Zheng B, Yin WN, Suzuki T, Zhang XH, Zhang Y, Song LL, Jin LS, Zhan H, Zhang H, Li JS, Wen JK. Exosome-Mediated miR-155 Transfer from Smooth Muscle Cells to Endothelial Cells Induces Endothelial Injury and Promotes Atherosclerosis. Mol. Ther. 2017;25(6):1279-1294. https://doi.org/10.1016/j.ymthe.2017.03.031

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Gurunathan S, Kang MH, Jeyaraj M, Qasim M, Kim JH. Review of the Isolation, Characterization, Biological Function, and Multifarious Therapeutic Approaches of Exosomes. Cells. 2019;8(4):307. https://doi.org/10.3390/cells8040307

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Barile L, Moccetti T, Marbán E, Vassalli G. Roles of exosomes in cardioprotection. Eur. Heart J. 2017;38(18):1372-1379. https://doi.org/10.1093/eurheartj/ehw304

    Article  CAS  PubMed  Google Scholar 

  7. Burger D, Viñas JL, Akbari S, Dehak H, Knoll W, Gutsol A, Carter A, Touyz RM, Allan DS, Burns KD. Human endothelial colony-forming cells protect against acute kidney injury: role of exosomes. Am. J. Pathol. 2015;185(8):2309-2323. https://doi.org/10.1016/j.ajpath.2015.04.010

    Article  CAS  PubMed  Google Scholar 

  8. Hergenreider E, Heydt S, Tréguer K, Boettger T, Horrevoets AJ, Zeiher AM, Scheffer MP, Frangakis AS, Yin X, Mayr M, Braun T, Urbich C, Boon RA, Dimmeler S. Atheroprotective communication between endothelial cells and smooth muscle cells through miRNAs. Nat. Cell Biol. 2012;14(3):249-256. https://doi.org/10.1038/ncb2441

    Article  CAS  PubMed  Google Scholar 

  9. Virmani R, Liistro F, Stankovic G, Di Mario C, Montorfano M, Farb A, Kolodgie FD, Colombo A. Mechanism of late in-stent restenosis after implantation of a paclitaxel derivate-eluting polymer stent system in humans. Circulation. 2002;106(21):2649-2651. https://doi.org/10.1161/01.cir.0000041632.02514.14

    Article  CAS  PubMed  Google Scholar 

  10. Huang C, Han J, Wu Y, Li S, Wang Q, Lin W, Zhu J. Exosomal MALAT1 derived from oxidized low-density lipoprotein-treated endothelial cells promotes M2 macrophage polarization. Mol. Med. Rep. 2018;18(1):509-515. https://doi.org/10.3892/mmr.2018.8982

    Article  CAS  PubMed  Google Scholar 

  11. Paredes F, Williams HC, Quintana RA, San Martin A. Mitochondrial Protein Poldip2 (Polymerase Delta Interacting Protein 2) Controls Vascular Smooth Muscle Differentiated Phenotype by O-Linked GlcNAc (N-Acetylglucosamine) Transferase-Dependent Inhibition of a Ubiquitin Proteasome System. Circ. Res. 2020;126(1):41-56. https://doi.org/10.1161/CIRCRESAHA.119.315932

    Article  CAS  PubMed  Google Scholar 

  12. Sun HJ, Zhao MX, Ren XS, Liu TY, Chen Q, Li YH, Kang YM, Wang JJ, Zhu GQ. Salusin-β Promotes Vascular Smooth Muscle Cell Migration and Intimal Hyperplasia After Vascular Injury via ROS/NFκB/MMP-9 Pathway. Antioxid. Redox Signal. 2016;24(18):1045-57. https://doi.org/10.1089/ars.2015.6475

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Cardiology, Affiliated Hospital of Zunyi Medical University, Zunyi, China

    S. Y. Yang, W. W. Deng, R. Z. Zhao, X. P. Long, D. M. Wang, H. H. Guo, L. X. Jiang, W. M. Chen & B. Shi

Authors
  1. S. Y. Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. W. Deng
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Z. Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. X. P. Long
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. D. M. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. H. H. Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. L. X. Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. W. M. Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. B. Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to B. Shi.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 174, No. 12, pp. 736-742, December, 2022

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yang, S.Y., Deng, W.W., Zhao, R.Z. et al. Exosomes Derived from Endothelial Cells Inhibit Neointimal Hyperplasia Induced by Carotid Artery Injury in Rats via ROS-NLRP3 Inflammasome Pathway. Bull Exp Biol Med 174, 762–767 (2023). https://doi.org/10.1007/s10517-023-05788-0

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-023-05788-0

Keywords

Search

Navigation