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Perfluorocarbon Protects against Lipopolysaccharide-Induced Apoptosis of Endothelial Cells in Pulmonary Microvessels

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This study was aimed to explore the effect and mechanisms of action of perfluorocarbon on LPS-induced apoptosis of pulmonary microvascular endothelial cells (PMVEC) isolated from Sprague-Dawley rats. Apoptosis rates were assessed by flow cytometry. Ultrastructural characteristics of PMVEC were evaluated by transmission electron microscopy. The protein expression of cleaved caspase-3 was measured using Western blotting. LPS significantly increased the level of apoptosis, induced the appearance of ultrastructural changes typical of apoptosis, up-regulated the expression of active caspase-3 protein. These effects of LPS were attenuated by co-administration of perfluorocarbon. These results suggest that perfluorocarbon can attenuate LPS-induced apoptosis of PMVEC by inhibiting TLR-4 signaling and caspase-3 activation.

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Authors and Affiliations

  1. Department of Respiratory Diseases, the First Medical Center, Chinese PLA General Hospital, Beijing, P.R. China

    Y. Xu, P. Wang & Z. X. Liang

  2. Department of Respiratory Diseases, the First Hospital of Qinhuangdao, Qinhuangdao, P.R. China

    T. Wu, S. S. Shi, S. F. Xu, X. J. Liu & Q. Tian

Authors
  1. Y. Xu
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  2. T. Wu
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  3. P. Wang
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  4. Z. X. Liang
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  5. S. S. Shi
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  6. S. F. Xu
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  7. X. J. Liu
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  8. Q. Tian
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Corresponding author

Correspondence to S. F. Xu.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 170, No. 10, pp. 415-419, October, 2020

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Xu, Y., Wu, T., Wang, P. et al. Perfluorocarbon Protects against Lipopolysaccharide-Induced Apoptosis of Endothelial Cells in Pulmonary Microvessels. Bull Exp Biol Med 170, 410–414 (2021). https://doi.org/10.1007/s10517-021-05077-8

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  • DOI: https://doi.org/10.1007/s10517-021-05077-8

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