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(–)-Epigallocatechin-3-O-gallate (EGCG) attenuates the hemodynamics stimulated by caffeine through decrease of catecholamines release

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Abstract

A human study of the effects on hemodynamics of caffeine and epigallocatechin-3-O-gallate (EGCG) was performed. Caffeine tablets (200 mg) were orally administered to healthy males aged between 25 and 35 years 30 min after oral administration of EGCG tablets (100 and 200 mg). The increase in BP induced by caffeine was inhibited when co-administrated with EGCG. We found that caffeine slightly decreased heart rate (HR) in the volunteers. Although EGCG enhanced HR reduction, the effect was not significant. In addition, caffeine increased blood catecholamine levels, but EGCG inhibited the increase in noradrenaline, adrenaline and dopamine levels induced by caffeine. Whether EGCG decreases the elevated HR and systolic perfusion pressure, and ventricular contractility induced by adrenergic agonists in the isolated rat heart was investigated. The modified Krebs–Henseleit solution was perfused through a Langendorff apparatus to the isolated hearts of rats. HR, systolic perfusion pressure, and developed maximal rates of contraction (+dP/dtmax) and relaxation (−dP/dtmax) were increased by epinephrine (EP) and isoproterenol (IP). In contrast, EGCG decreased the elevated HR, systolic perfusion pressure, and left ventricular ±dp/dtmax induced by EP and/or IP. In conclusion, EGCG could attenuate the hemodynamics stimulated by caffeine through decreasing catecholamine release.

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References

  • Adachi N, Tomonaga S, Tachibana T, Denbow DM, Furuse M (2006) (–)-Epigallocatechin gallate attenuates acute stress responses through GABAergic system in the brain. Eur J Pharmacol 531:171–175

    Article  CAS  PubMed  Google Scholar 

  • Adaramoye OA, Anjos RM, Almeida MM, Veras RC, Silvia DF, Oliveira FA, Cavalcante KV, Araujo IG, Oliveira AP, Medeiros IA (2009) Hypotensive and endothelium-independent vasorelaxant effects of methanolic extract from Curcuma longa L. in rats. J Ethnopharmacol 124:457–462

    Article  PubMed  Google Scholar 

  • Aggio A, Grassi D, Onori E, D’alessandro A, Masedu F, Valenti M, Ferri C (2013) Endothelium/nitric oxide mechanism mediates vasorelaxation and counteracts vasoconstriction induced by low concentration of flavanols. Eur J Nutr 52:263–272

    Article  CAS  PubMed  Google Scholar 

  • Alvarez E, Campos-Toimil M, Justiniano-Basaran H, Lugnier C, Orallo F (2006) Study of the mechanisms involved in the vasorelaxation induced by (–)-epigallocatechin-3-gallate in rat aorta. Br J Pharmacol 147:269–280

    Article  CAS  PubMed  Google Scholar 

  • Avidor T, Clementi E, Schwartz L, Atlas D (1994) Caffeine-induced transmitter release is mediated via ryanodine-sensitive channel. Neurosci Lett 165:133–136

    Article  CAS  PubMed  Google Scholar 

  • Castro J, Pregibon T, Chumanov K, Marcus RK (2010) Determination of catechins and caffeine in proposed green tea standard reference materials by liquid chromatography-particle beam/electron ionization mass spectrometry (LC-PB/EIMS). Talanta 82:1687–1695

    Article  CAS  PubMed  Google Scholar 

  • Chen ZY, Yao XQ, Chan FL, Lau CW, Huang Y (2002) (–)epicatechin induces and modulates endothelium-dependent relaxation in isolated rat mesenteric artery rings. Acta Pharmacol Sin 23:1188–1192

    CAS  PubMed  Google Scholar 

  • Chen Q, Guo Z, Zhao J (2008) Identification of green tea’s (Camellia sinensis (L.)) quality level according to measurement of main catechins and caffeine contents by HPLC and support vector classification pattern recognition. J Pharm Biomed Anal 48:1321–1325

    Article  CAS  PubMed  Google Scholar 

  • Daly JW, Bruns RF, Snyder SH (1981) Adenosine receptors in the central nervous system: relationship to the central actions of methylxanthines. Life Sci 28:2083–2097

    Article  CAS  PubMed  Google Scholar 

  • Daul A, Hermes U, Schafers RF, Wenzel R, Von Birgelen C, Brodde OE (1995) The beta-adrenoceptor subtype(s) mediating adrenaline- and dobutamine-induced blood pressure and heart rate changes in healthy volunteers. Int J Clin Pharmacol Ther 33:140–148

    CAS  PubMed  Google Scholar 

  • Devika PT, Mainzen Prince PS (2008) (–)-Epigallocatechin gallate (EGCG) prevents isoprenaline-induced cardiac marker enzymes and membrane-bound ATPases. J Pharm Pharmacol 60:125–133

    Article  CAS  PubMed  Google Scholar 

  • Fredholm BB (1984) Cardiovascular and renal actions of methylxanthines. Prog Clin Biol Res 158:303–330

    CAS  PubMed  Google Scholar 

  • Grover GJ, Mccullough JR, D’alonzo AJ, Sargent CA, Atwal KS (1995) Cardioprotective profile of the cardiac-selective ATP-sensitive potassium channel opener BMS-180448. J Cardiovasc Pharmacol 25:40–50

    Article  CAS  PubMed  Google Scholar 

  • Guo L, Dong Z, Guthrie H (2009) Validation of a guinea pig Langendorff heart model for assessing potential cardiovascular liability of drug candidates. J Pharmacol Toxicol Methods 60:130–151

    Article  CAS  PubMed  Google Scholar 

  • Han JY, Kim CS, Lim KH, Kim JH, Kim S, Yun YP, Hong JT, Oh KW (2011) Increases in blood pressure and heart rate induced by caffeine are inhibited by (–)-epigallocatechin-3-O-gallate: involvement of catecholamines. J Cardiovasc Pharmacol 58:446–449

    CAS  PubMed  Google Scholar 

  • He YH, Kies C (1994) Green and black tea consumption by humans: impact on polyphenol concentrations in feces, blood and urine. Plant Foods Hum Nutr 46:221–229

    Article  CAS  PubMed  Google Scholar 

  • Kim SY, Kim DS, Kwon SB, Park ES, Huh CH, Youn SW, Kim SW, Park KC (2005) Protective effects of EGCG on UVB-induced damage in living skin equivalents. Arch Pharm Res 28:784–790

    Article  CAS  PubMed  Google Scholar 

  • Lim DY (2005) Comparison of green tea extract and epigallocatechin gallate on secretion of catecholamines from the rabbit adrenal medulla. Arch Pharm Res 28:914–922

    Article  CAS  PubMed  Google Scholar 

  • Mcmullen MK, Whitehouse JM, Shine G, Whitton PA, Towell A (2012) Caffeine in hot drinks elicits cephalic phase responses involving cardiac activity. Food Funct 3:931–940

    Article  CAS  PubMed  Google Scholar 

  • Michel MC (1993) Adrenoreceptor subtypes–function and significance. Med Monatsschr Pharm 16:130–136

    CAS  PubMed  Google Scholar 

  • Park KS, Eun JS, Kim HC, Moon DC, Hong JT, Oh KW (2010) (–)-Epigallocatethin-3-O-gallate counteracts caffeine-induced hyperactivity: evidence of dopaminergic blockade. Behav Pharmacol 21:572–575

    Article  CAS  PubMed  Google Scholar 

  • Riksen NP, Smits P, Rongen GA (2011) The cardiovascular effects of methylxanthines. Handb Exp Pharmacol 200:413–437

    Article  CAS  PubMed  Google Scholar 

  • Wang X, Hao MW, Dong K, Lin F, Ren JH, Zhang HZ (2009) Apoptosis induction effects of EGCG in laryngeal squamous cell carcinoma cells through telomerase repression. Arch Pharm Res 32:1263–1269

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MSIP) (MRC, 2008-0062275).

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Authors and Affiliations

  1. College of Veterinary Medicine, Chungbuk National University, Cheongju, 362-763, Republic of Korea

    Jin-Yi Han

  2. College of Oriental Medicine, Wonkwang University, Iksan, 579-749, Republic of Korea

    Yong-Jin Moon & Jong-Hyun Han

  3. College of Veterinary Medicine, Chonbuk National University, Jeonju, 579-749, Republic of Korea

    Jong-Hoon Kim

  4. College of Pharmacy and Medical Research Center (MRC), Chungbuk National University, Cheongju, 28644, Republic of Korea

    Jae-Hoon Woo, Hwan-Soo Yoo, Jin Tae Hong & Ki-Wan Oh

  5. College of Medicine, Chungbuk National University, Cheongju, 28644, Republic of Korea

    Hee-Yul Ahn & Jong-Myeon Hong

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  1. Jin-Yi Han
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  2. Yong-Jin Moon
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  3. Jong-Hyun Han
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Correspondence to Jong-Myeon Hong or Ki-Wan Oh.

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Han, JY., Moon, YJ., Han, JH. et al. (–)-Epigallocatechin-3-O-gallate (EGCG) attenuates the hemodynamics stimulated by caffeine through decrease of catecholamines release. Arch. Pharm. Res. 39, 1307–1312 (2016). https://doi.org/10.1007/s12272-016-0757-1

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  • DOI: https://doi.org/10.1007/s12272-016-0757-1

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