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In Vivo Effects of Human Bone Marrow Mesenchymal Stromal Cells on the Development of Experimental B16 Melanoma in Mice

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Experiments on F1(CBA×C57BL/6) mice with experimental metastatic melanoma B16 F10 showed that single intravenous injection of xenogeneic bone marrow mesenchymal stromal cells (BM-MSC) in a dose of 106 cells/mouse significantly increased 100-day survival rate of tumor-bearing animals. In contrast, administration of BM-MSC in a dose of 2×106 cells/ mouse reduced survival rates in comparison with the biocontrol (injection of B16 cells alone, 5×105 cells/mouse). This phenomenon can be related to in vivo participation of BM-MSC in reprogramming of resident tissue macrophages, including tumor microenvironment, towards pro- (M1) or anti-inflammatory (M2) phenotype. This is indirectly confirmed by the data on switching from activation to inhibition of ROS-producing activity of blood mononuclears and peritoneal macrophages in tumor-bearing mice in the test of luminol-dependent zymosaninduced chemiluminescence.

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Authors and Affiliations

  1. A. F. Tsyb Medical Radiological Research Center, Affiliated Branch of the National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Obninsk, Russia

    V. N. Petrov, E. V. Isaeva, S. E. Ulyanenko, E. E. Beketov, E. M. Yatsenko, E. V. Sayapina, L. A. Lepekhina, N. V. Nasedkina, L. Yu. Grivtsova & A. D. Kaprin

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  1. V. N. Petrov
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  2. E. V. Isaeva
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  3. S. E. Ulyanenko
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  4. E. E. Beketov
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  5. E. M. Yatsenko
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  6. E. V. Sayapina
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  7. L. A. Lepekhina
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  8. N. V. Nasedkina
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  9. L. Yu. Grivtsova
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  10. A. D. Kaprin
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Correspondence to E. V. Isaeva.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 248-252, December, 2019

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Petrov, V.N., Isaeva, E.V., Ulyanenko, S.E. et al. In Vivo Effects of Human Bone Marrow Mesenchymal Stromal Cells on the Development of Experimental B16 Melanoma in Mice. Bull Exp Biol Med 168, 561–565 (2020). https://doi.org/10.1007/s10517-020-04753-5

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  • DOI: https://doi.org/10.1007/s10517-020-04753-5

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