In vivo modifying effects of bone marrow mesenchymal stromal cells of humans and laboratory mice on ROS production by mouse blood mononuclears are studied by luminol-dependent zymosan-induced chemiluminescence after syngeneic and xenogeneic transplantation into systemic blood flow. The chemiluminescent activity of mouse blood mononuclears has increased early (1 day) after syngeneic (mouse mesenchymal stromal cells) and xenogeneic (human mesenchymal stromal cells) transplantation. Later, 7-21 days after syngeneic and xenogeneic transplantation, the chemiluminescent activity of mouse mononuclears is suppressed. The probable mechanisms of involvement of the transplanted mesenchymal stromal cells in reprogramming of the blood mononuclear phagocytes from proinflammatory (M1) to anti-inflammatory (M2) phenotype under conditions of their in vivo interactions are discussed; a frequent manifestation of this reprogramming is transition of the phase of activation into inhibition of ROS-producing activity of macrophages.
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References
Andreeva ER, Buravkova LB. Multipotent mesenchymal stromal and immune cells interaction: reciprocal effects. Ross. Fiziol. Zh. 2012;98(12):1441-1459. Russian.
Mayanskaya IV, Goganova AYu, Tolkachova NI, Achkinazi VI, Mayansky AN. Immunosuppressive activity of mesenchymal stem (stromal) cells. Immunologiya. 2013;34(2):122-128. Russian.
Tsyb AF, Petrov VN, Konoplyannikov AG, Saypina EV, Lepechina LA, Kalsina SSh, Semenkova IV, Agaeva EV. In vitro inhibitory effect of mesenchymal stem cells on zymosan-induced production of reactive oxygen species. Bull. Exp. Biol. Med. 2008;146(1):158-164.
Cassatella MA, Mosna F, Micheletti A, Lisi V, Tamassia N, Cont C, Calzetti F, Pelletier M, Pizzolo G, Krampera M. Tolllike receptor-3-activated human mesenchymal stromal cells significantly prolong the survival and function of neutrophils. Stem Cells. 2011;29(6):1001-1011.
Choi H, Lee RH, Bazhanov N, Oh JY, Prockop DJ. Antiinflammatory protein TSG-6 secreted by activated MSCs attenuates zymosan-induced mouse peritonitis by decreasing TLR2/NF-κB signaling in resident macrophages. Blood. 2011;118(2):330-338.
Glenn JD, Whartenby KA. Mesenchymal stem cells: Emerging mechanisms of immunomodulation and therapy. World J. Stem Cells. 2014;6(5):526-539.
Kim J, Hematti P. Mesenchymal stem cell-educated macrophages: a novel type of alternatively activated macrophages. Exp. Hematol. 2009;37(12):1445-1453.
Leibacher J, Henschler R. Biodistribution, migration and homing of systemically applied mesenchymal stem/stromal cells. Stem Cell Res. Ther. 2016;7:7. doi: https://doi.org/10.1186/s13287-015-0271-2.
Mesenchymal Stem Cells. Methods and protocols / M.Gnecchi N.Y, 2016.
Molina E.R, Smith B.T, Shah S.R, Shin H, Mikos A.G. Immunomodulatory properties of stem cells and bioactive molecules for tissue engineering. J. Control. Release. 2015;219:107-118.
Raffaghello L, Bianchi G, Bertolotto M, Montecucco F, Busca A, Dallegri F, Ottonello L, Pistoia V. Human mesenchymal stem cells inhibit neutrophil apoptosis: a model for neutrophil preservation in the bone marrow niche. Stem Cells. 2008;26(1):151-162.
Sharma R. R, Pollock K, Hubel A, McKenna D. Mesenchymal stem or stromal cells: a review of clinical applications and manufacturing practices. Transfusion. 2014;54(5):1418-1437.
Wang N, Liang H, Zen K. Molecular mechanisms that influence the macrophage m1-m2 polarization balance. Front. Immunol. 2014;5:614. doi: https://doi.org/10.3389/fimmu.2014.00614.
Waterman R.S, Tomchuck S.L, Henkle S.L, Betancourt A.M. A new Mesenchymal Stem Cell (MSC) paradigm: polarization into a pro-inflammotory MSC1 or an immunosuppressive MSC2 phenotype. PLoS One. 2010;5(4):e10088. doi: https://doi.org/10.1371/journal. pone.0010088.
Zheng G, Ge M, Qiu G, Shu Q, Xu J. Mesenchymal stromal cells affect disease outcomes via macrophage polarization. Stem Cells Int. 2015;2015. ID 989473. doi: 10.1155/2015/989473.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 163, No. 7, pp. 95-99, July, 2017
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Agaeva, E.V., Petrov, V.N., Konoplyannikov, A.G. et al. Syngeneic and Xenogeneic Transplantations of Mesenchymal Stromal Cells Modify the Production of Reactive Oxygen Species by Blood Mononuclears of Mice. Bull Exp Biol Med 164, 80–84 (2017). https://doi.org/10.1007/s10517-017-3929-1
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DOI: https://doi.org/10.1007/s10517-017-3929-1