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Role of MAPK ERK1/2 and p38 in the Realization of Growth Potential of Various Types of Regeneration-Competent Cells in Mouse Neural Tissue during Ethanol-Induced Neurodegeneration In Vitro

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Ethanol-induced neurodegeneration was modeled in vitro to study the roles of ERK1/2 and p38 in realization of the growth potential of neural progenitor cells and secretion of neurotrophic growth factors by glial elements. Addition of the neurotoxic dose of C2H5OH (65 mM) to the culture medium abolished the effects of specific ERK1/2 and p38 inhibitors on the formation of colonies (neurospheres) and proliferative activity of neural CFU in cultured cells derived from paraventricular region of the mouse brain. The study established that these protein kinases are not implicated in ethanol-induced stimulation of the formation of neural CFU, differentiation of neural progenitors, and synthesis of humoral functional regulators of neural CFU by glial cells.

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References

  1. Dygai AM, Zyuz’kov GN, Zhdanov VV, Udut EV, Khrichkova TYu, Miroshnichenko LA, Simanina EV, Stavriva LA. Methodical recommendations for studying specific activity of agents for regenerative medicine. Manual for Preclinical Studies of New Pharmacological Substances. Mironov AN, ed. Moscow, 2013. Part I. P. 776-787. Russian.

  2. Zyuz’kov GN, Miroshnichenko LA, Udut EV, Chaikovskii AV, Polyakova TY, Simanina EV, Stavrova LA, Agafonov VI, Zhdanov VV. Functional State of Various Types of Regeneration-Competent Cells in the Nervous Tissue in Ethanol-Induced Neurodegeneration. Bull. Exp. Biol. Med. 2019;166(3):317-320.

    Article  PubMed  Google Scholar 

  3. Zyuz’kov GN, Zhdanov VV, Udut EV, Miroshnichenko LA, Polyakova TYu, Stavrova LA, Udut VV. Strategy of Pharmacological Regulation of Intracellular Signal Transduction in Regeneration-Competent Cells. Bull. Exp. Biol. Med. 2019;166(4):435-445.

    Article  Google Scholar 

  4. Zyuzkov GN, Zhdanov VV, Danilets MG, Miroshnichenko LA, Udut EV, Dygaj AM. Patent RU No. 2599289. Tissue regeneration stimulating agent. Bull. No. 28. Published October 10, 2016.

  5. Brust JC. Acute withdrawal: diagnosis and treatment. Handb. Clin. Neurol. 2014;125:123-131.

    Article  PubMed  Google Scholar 

  6. Crews FT, Nixon K. Alcohol, neural stem cells, and adult neurogenesis. Alcohol Res. Health. 2003;27(2):197-204.

    PubMed  PubMed Central  Google Scholar 

  7. Jang MH, Shin MC, Jung SB, Lee TH, Bahn GH, Kwon YK, Kim EH, Kim CJ. Alcohol and nicotine reduce cell proliferation and enhance apoptosis in dentate gyrus. Neuroreport. 2002;13(12):1509-1513.

    Article  CAS  PubMed  Google Scholar 

  8. Jordaan GP, Emsley R. Alcohol-induced psychotic disorder: a review. Metab. Brain Dis. 2014;29(2):231-243.

    Article  CAS  PubMed  Google Scholar 

  9. Nilsson M, Sonne C. Diagnostics and treatment of Wernicke—Korsakoff syndrome patients with an alcohol abuse. Ugeskr. Laeger. 2013;175(14):942-944.

    PubMed  Google Scholar 

  10. Nixon K. Alcohol and adult neurogenesis: roles in neurodegeneration and recovery in chronic alcoholism. Hippocampus. 2006;16(3):287-295.

    Article  CAS  PubMed  Google Scholar 

  11. Pearson G, Robinson F, Beers Gibson T, Xu B. E, Karandikar M, Berman K, Cobb M.H. Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions. Endocr. Rev. 2001;22(2):153-183.

    CAS  PubMed  Google Scholar 

  12. Tateno M, Ukai W, Yamamoto M, Hashimoto E, Ikeda H, Saito T. The effect of ethanol on cell fate determination of neural stem cells. Alcohol. Clin. Exp. Res. 2005;29(12, Suppl):225S-229S.

    Article  PubMed  Google Scholar 

  13. Tateno M, Saito T. Biological Studies on Alcohol-Induced Neuronal Damage. Psychiatry Investig. 2008;5(1):21-27.

    Article  PubMed  PubMed Central  Google Scholar 

  14. Thórarinsson BL, Ólafsson E, Kjartansson Ó, Blöndal H. Wernicke’s encephalopathy in chronic alcoholics. Laeknabladid. 2011;97(1):21-29.

    PubMed  Google Scholar 

  15. Yang JY, Xue X, Tian H, Wang XX, Dong YX, Wang F, Zhao YN, Yao XC, Cui W, Wu CF. Role of microglia in ethanolinduced neurodegenerative disease: Pathological and behavioral dysfunction at different developmental stages. Pharmacol. Ther. 2014;144(3):321-337.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Tomsk, Russia

    G. N. Zyuz’kov, L. A. Miroshnichenko, T. Yu. Polyakova, L. A. Stavrova, E. V. Simanina, V. I. Agafonov, E. V. Udut & V. V. Zhdanov

Authors
  1. G. N. Zyuz’kov
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  2. L. A. Miroshnichenko
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  3. T. Yu. Polyakova
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  4. L. A. Stavrova
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  5. E. V. Simanina
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  6. V. I. Agafonov
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  7. E. V. Udut
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  8. V. V. Zhdanov
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Corresponding author

Correspondence to G. N. Zyuz’kov.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 167, No. 2, pp. 179-183, February, 2019

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Zyuz’kov, G.N., Miroshnichenko, L.A., Polyakova, T.Y. et al. Role of MAPK ERK1/2 and p38 in the Realization of Growth Potential of Various Types of Regeneration-Competent Cells in Mouse Neural Tissue during Ethanol-Induced Neurodegeneration In Vitro. Bull Exp Biol Med 167, 229–232 (2019). https://doi.org/10.1007/s10517-019-04497-x

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  • DOI: https://doi.org/10.1007/s10517-019-04497-x

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