We studied changes in the expression of mRNA for mucins and claudins in the medial part of the colon in male C57Bl/6 mice on the model of acute and chronic colitis induced by substitution of drinking water with 1% solution of dextran sodium sulphate for 5 days. In acute colitis, the expression of the main structural component of glycocalyx, mucin Muc3, decreased and expression of pore-forming claudin Cldn2 increased, which reflected enhanced permeability of tight junctions. In the chronic colitis group, in comparison with the normal group, we observed an increase in expression of mRNA of main structural mucus component Muc2, enhanced of expression of Muc1 associated with carcinogenesis, and reduced expression of Muc13, which led to a more severe course of colitis; the expression of pore-forming claudin Cldn2 was elevated. These findings indicate that the imbalance in the expression of mucins and claudins plays an important role in the mechanisms of development of acute and chronic colitis.
Similar content being viewed by others
References
Zolotova NA, Makarova OV. Barrier function of colonic mucins in health and ulcerative colitis. Klin. Eksp. Morfol. 2016;19(3):69-74. Russian.
Zolotova NA, Khochansky DN, Polikarpova AV, Dobrinina MT, Makarova OV. Cytological and physiological characteristics of epithelial sec retory cells from different parts of the colon in experimental ulcerative colitis. Klin. Eksp. Morfol. 2016;18(2):29-37. Russian.
Ivashkin VT, Shelygin YuA, Khalif IL, Belousova EA, Shifrin OS, Abdulganieva DI, Abdulkhakov RA, Alekseeva OP, Alekseenko SA, Achkasov SI, Baranovsky AYu, Bolikhov KV, Valuiskikh EYu, Vardanyan AV, Veselov AV, Veselov VV, Golovenko AO, Golovenko OV, Grigorev EG, Gubonina IV, Zhigalova TN, Kashnikov VN, Knyazev OV, Kostenko NV, Kulyapin AV, Morozova NA, Muravev AV, Nizov AA, Nikitina NV, Nikolaeva NN, Osipenko MF, Pavlenko VV, Parfenov AI, Poluektova EA, Potapov AS, Rumyantsev VG, Svetlova IO, Sitkin SI, Timerbulatov VM, Tkachev AV, Tkachenko EI, Frolov SA, Khubezov DA, Chashkova EYu, Shapina MV, Shchukina OB, Yakovlev AA. Clinical guide of Russian Association of Gastroenterology and Russian Association of Coloproctology on diagnostics and treatment of ulcerative colitis. Kolproktologiya. 2017;(1):6-30. Russian.
Tkachev AV, Mkrtchyan LS, Nikitina KE, Volynskaya EI. Inflammatory bowel disease: crossing of the problems. Prakt. Med. 2012;(3):17-22. Russian.
Antoni L, Nuding S, Wehkamp J, Stange EF. Intestinal barrier in inflammatory bowel disease. World J. Gastroenterol. 2014;20(5):1165-1179.
Günzel D, Yu AS. Claudins and the modulation of tight junction permeability. Physiol. Rev. 2013;93(2):525-569.
Hoebler C, Gaudier E, De Coppet P, Rival M, Cherbut C. MUC genes are differently expressed during onset and maintenance of inflammation in dextran sodium sulfate-treated mice. Dig. Dis. Sci. 2006;51(2):381-389.
Longman RJ, Poulsom R, Corfield AP, Warren BF, Wright NA, Thomas MG. Alterations in the composition of the supramucosal defense barrier in relation to disease severity of ulcerative colitis. J. Histochem. Cytochem. 2006;54(12):1335-1348.
Mennigen R, Nolte K, Rijcken E, Utech M, Loeffler B, Senninger N, Bruewer M. Probiotic mixture VSL#3 protects the epithelial barrier by maintaining tight junction protein expression and preventing apoptosis in a murine model of colitis. Am. J. Physiol. Gastrointest. Liver Physiol. 2009;296(5):G1140-G1149.
Moehle C, Ackermann N, Langmann T, Aslanidis C, Kel A, Kel-Margoulis O, Schmitz-Madry A, Zahn A, Stremmel W, Schmitz G. Aberrant intestinal expression and allelic variants of mucin genes associated with inflammatory bowel disease. J. Mol. Med. (Berl). 2006;84(12):1055-1066.
Oshima T, Miwa H, Joh T. Changes in the expression of claudins in active ulcerative colitis. J. Gastroenterol. Hepatol. 2008;23(Suppl 2):S146-S150.
Randhawa PK, Singh K, Singh N, Jaggi AS. A review on chemical-induced inflammatory bowel disease models in rodents. Korean J. Physiol. Pharmacol. 2014;18(4):279-288.
Sheng YH, Lourie R, Lindén SK, Jeffery PL, Roche D, Tran TV, Png CW, Waterhouse N, Sutton P, Florin TH, McGuckin MA. The MUC13 cell-surface mucin protects against intestinal inflammation by inhibiting epithelial cell apoptosis. Gut. 2011;60(12):1661-1670.
Yuan B, Zhou S, Lu Y, Liu J, Jin X, Wan H, Wang F. Changes in the expression and distribution of claudins, increased epithelial apoptosis, and a mannan-binding lectin-associated immune response lead to barrier dysfunction in dextran sodium sulfate-induced rat colitis. Gut Liver. 2015;9(6):734-740.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 165, No. 4, pp. 421-424, April, 2018
Rights and permissions
About this article
Cite this article
Zolotova, N.A., Polikarpova, A.V., Khochanskii, D.N. et al. Expression of Mucins and Claudins in the Colon during Acute and Chronic Experimental Colitis. Bull Exp Biol Med 165, 434–437 (2018). https://doi.org/10.1007/s10517-018-4187-6
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-018-4187-6