We studied the relationship between diffusion transport and morphological and microstructural organization of extracellular matrix of human intervertebral disk. Specimens of the lumbar intervertebral disks without abnormalities were studied ex vivo by diffusion-weighed magnetic resonance imaging, histological and immunohistochemical methods, and electron microscopy. Distribution of the diffusion coefficient in various compartments of the intervertebral disk was studied. Significant correlations between diffusion coefficient and cell density in the nucleus pulposus, posterior aspects of annulus fibrosus, and endplate at the level of the posterior annulus fibrosus were detected for each disk. In disks with nucleus pulposus diffusion coefficient below 15×10-4 mm2/sec, collagens X and XI were detected apart from aggrecan and collagens I and II. The results supplement the concept on the relationship between the microstructure and cell composition of various compartments of the intervertebral disk and parameters of nutrient transport.
Similar content being viewed by others
References
Belykh E, Giers M, Bardonova L, Theodore N, Preul M, Byvaltsev V. The Role of bone morphogenetic proteins 2, 7, and 14 in approaches for intervertebral disk restoration. World Neurosurg. 2015;84(4):871-873.
Byvaltsev VA, Kalinin AA, Stepanov IA, Shashkov KV. Diffusion-weighted magnetic resonance tomography in the diagnosis of intervertebral disk degeneration. Biomed. Engineering. 2016;50(4):253-256.
Giers MB, Munter BT, Eyster KJ, Ide GD, Newcomb AGUS, Lehrman JN, Belykh E, Byvaltsev VA, Kelly BP, Preul MC, Theodore N. Biomechanical and endplate effects on nutrient transport in the intervertebral disc. World Neurosurg. 2017;99:395-402.
Kealey SM, Aho T, Delong D, Barboriak DP, Provenzale JM, Eastwood JD. Assessment of apparent diffusion coefficient in normal and degenerated intervertebral lumbar disks: initial experience. Radiology. 2005;235(2):569-574.
Manek NJ, MacGregor AJ. Epidemiology of back disorders: prevalence, risk factors, and prognosis. Curr. Opin. Rheumatol. 2005;17(2):134-140.
Niinimäki J, Korkiakoski A, Ojala O, Karppinen J, Ruohonen J, Haapea M, Korpelainen R, Natri A, Tervonen O. Association between visual degeneration of intervertebral discs and the apparent diffusion coefficient. Magn. Reson. Imaging. 2009;27(5):641-647.
Pfirrmann CW, Metzdorf A, Zanetti M, Hodler J, Boos N. Magnetic resonance classification of lumbar intervertebral disc degeneration. Spine (Phila Pa 1976). 2001;26(17):1873-1878.
Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, Preibisch S, Rueden C, Saalfeld S, Schmid B, Tinevez JY, White DJ, Hartenstein V, Eliceiri K, Tomancak P, Cardona A. Fiji: an open-source platform for biological-image analysis. Nat. Methods. 2012;9(7):676-682.
Tomaszewski KA, Walocha JA, Mizia E, Gładysz T, Głowacki R, Tomaszewska R. Age- and degeneration-related variations in cell density and glycosaminoglycan content in the human cervical intervertebral disc and its endplates. Pol. J. Pathol. 2015;66(3):296-309.
Wang AM, Cao P, Yee A, Chan D, Wu EX. Detection of extracellular matrix degradation in intervertebral disc degeneration by diffusion magnetic resonance spectroscopy. Magn. Reson. Med. 2015;73(5):1703-1712.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 164, No. 8, pp. 255-260, August, 2017
Rights and permissions
About this article
Cite this article
Byvaltsev, V.A., Kolesnikov, S.I., Belykh, E.G. et al. Complex Analysis of Diffusion Transport and Microstructure of an Intervertebral Disk. Bull Exp Biol Med 164, 223–228 (2017). https://doi.org/10.1007/s10517-017-3963-z
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-017-3963-z