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The Influence of Proinflammatory Factors on the Neuroprotective Efficiency of Multipotent Mesenchymal Stromal Cells in Traumatic Brain Injury

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

We studied the neuroprotective potential of multipotent mesenchymal stromal cells in traumatic brain injury and the effect of inflammatory preconditioning on neuroprotective properties of stem cells under in vitro conditions. To this end, the effects of cell incubation with LPS or their co-culturing with leukocytes on production of cytokines IL-1α, IL-6, TNFα, and MMP-2 and MMP-9 by these cells were evaluated. Culturing under conditions simulating inflammation increased the production of all these factors by multipotent mesenchymal stromal cells. However, acquisition of the inflammatory phenotype by stromal cells did not reduce their therapeutic effectiveness in traumatic brain injury. Moreover, in some variants of inflammatory preconditioning, multipotent mesenchymal stromal cells exhibited more pronounced neuroprotective properties reducing the volume of brain lesion and promoting recovery of neurological functions after traumatic brain injury.

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Authors and Affiliations

  1. Faculty of Bioengineering and Bioinformatics, M. V. Lomonosov Moscow State University, Moscow, Russia

    T. I. Danilina

  2. A. N. Belozersky Institute of Physicochemical Biology, M. V. Lomonosov Moscow State University, Moscow, Russia

    D. N. Silachev, I. B. Pevzner, L. D. Zorova, E. Yu. Plotnikov & D. B. Zorov

  3. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia

    D. N. Silachev, I. B. Pevzner, L. D. Zorova, E. Yu. Plotnikov, G. T. Sukhikh & D. B. Zorov

  4. Laboratory of Magnetic Tomography and Spectroscopy, Faculty of Fundamental Medicine, M. V. Lomonosov Moscow State University, Moscow, Russia

    M. V. Gulyaev & Yu. A. Pirogov

  5. International Laser Research Center, M. V. Lomonosov Moscow State University, Moscow, Russia

    L. D. Zorova

Authors
  1. T. I. Danilina
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  2. D. N. Silachev
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  3. I. B. Pevzner
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  4. M. V. Gulyaev
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  5. Yu. A. Pirogov
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  6. L. D. Zorova
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  7. E. Yu. Plotnikov
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  8. G. T. Sukhikh
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  9. D. B. Zorov
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Corresponding author

Correspondence to E. Yu. Plotnikov.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 2, pp. 77-84, June, 2017

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Danilina, T.I., Silachev, D.N., Pevzner, I.B. et al. The Influence of Proinflammatory Factors on the Neuroprotective Efficiency of Multipotent Mesenchymal Stromal Cells in Traumatic Brain Injury. Bull Exp Biol Med 163, 528–534 (2017). https://doi.org/10.1007/s10517-017-3844-5

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  • DOI: https://doi.org/10.1007/s10517-017-3844-5

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