Skip to main content
SpringerLink
Log in

Protective Activity of a Glycoconjugate Based on a Synthetic Hexasaccharide Related to a Fragment of Capsular Streptococcus Pneumoniae Serotype 14 Polysaccharide Chain

  • Microbiology and Immunology
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

We studied the effects of immunization with a conjugate of carrier protein and hexasaccharide ligand related to a fragment of capsular of Str. pneumoniae serotype 14 polysaccharide chain on activation of innate and adaptive immunity. It was found that two-fold immunization with the glycoconjugate adsorbed on aluminum hydroxide significantly increased the titer of IgG antibodies to capsular polysaccharide in the blood and protected 100% mice from infection with Str. pneumoniae serotype 14. Enhanced bactericidal activity of peripheral blood lymphocytes of mice was found 4 and 24 h after the first immunization with the immobilized glycoconjugate. Adsorption of the glycoconjugate on aluminum hydroxide resulted in modification of the immune processes at the stage of activation of innate immunity and subsequent strengthening of the adaptive immunity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. D. S. Vorob’ev, I. B. Semenova, Yu. V. Volokh, et al., Zh. Mikrobiol. Epidemiol. Immunol., No. 1, 21-26 (2013).

  2. R. S. Kozlov, A. N. Chagaryan, L. V. Kozlova, and A. A. Murav’ev, Klin. Mikrobiol. Antimikrob. Khimioter., 13, No. 2, 177-187 (2011).

    Google Scholar 

  3. E. A. Kurbatova, D. S. Vorob’ev, I. B. Semenova, et al., Biokhimiya, 78, No. 7, 1046-1052 (2013).

    Google Scholar 

  4. Resolution of Proceedings of Social Coordination Council on Pneumococcal Disease in Russia, Epidemiologiya Infektsionnie Bolezni. Aktual’nie Voprosi, No. 5, 82-83 (2012).

  5. S. M. Kharit, Vaccines and Vaccination: National Guideless [in Russian], Eds. V. V. Zverev, et al., Moscow (2011).

  6. T. Coffey, S. Berron, M. Daniels, et al., Microbiology, 142, Pt. 10, 2747-2757 (1996).

    Article  PubMed  CAS  Google Scholar 

  7. C. Hyams, J. Yuste, K. Bax, et al., Infect. Immun., 78, No. 2, 716-725 (2010).

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  8. W. T. Jansen, S. Hogenboom, M. J. Thijssen, et al., Infect. Immun., 69, No. 2, 787-93 (2001).

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  9. W. T. Jansen and H. Snippe, Indian J. Med. Res., 119, Suppl., 7-12 (2004).

    PubMed  CAS  Google Scholar 

  10. J. P. Kamerling, Streptococcus Pneumoniae Molecular Biology and Mechanism of Disease, Ed. A. Tomasz, New Rochelle, New York (2000), pp. 81-114.

  11. N. K. Kochetkov, N. E. Nifant’ev, and L. V. Backinowsky, Tetrahedron, 43, 3109-3121 (1987).

    Article  CAS  Google Scholar 

  12. N. E. Nifant’ev, L. V. Backinowsky, and N. K. Kochetkov, Carbohydr. Res., 174, 61-72 (1988).

    Article  Google Scholar 

  13. K. L. O’Brien, L. J. Wolfson, J. P. Watt, et al., Lancet, 374, 893-902 (2009).

    Article  PubMed  Google Scholar 

  14. D. Safari, H. A. Dekker, and J. A. Joosten, Infect. Immun., 76, No. 10, 4615-4623 (2008).

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  15. D. M. Weinberger, Z. B. Harboe, E. A. Sanders, et al., Clin. Infect. Dis., 51, No. 6, 692-699 (2010).

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. I. I. Mechnikov Research Institute of Vaccines and Sera, Russian Academy of Medical Sciences, Moscow, Russia

    E. A. Kurbatova, D. S. Vorob’ev, E. A. Akhmatov, N. K. Akhmatova, N. B. Egorova & N. E. Nifant’ev

  2. N. D. Zelinskii Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia

    Yu. E. Tsvetkov & E. V. Sukhova

  3. V. N. Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia

    D. V. Yashunskii

Authors
  1. E. A. Kurbatova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. S. Vorob’ev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. A. Akhmatov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. K. Akhmatova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. N. B. Egorova
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yu. E. Tsvetkov
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. E. V. Sukhova
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. D. V. Yashunskii
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. N. E. Nifant’ev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. A. Kurbatova.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 157, No. 5, pp. 630-633, May, 2014

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kurbatova, E.A., Vorob’ev, D.S., Akhmatov, E.A. et al. Protective Activity of a Glycoconjugate Based on a Synthetic Hexasaccharide Related to a Fragment of Capsular Streptococcus Pneumoniae Serotype 14 Polysaccharide Chain. Bull Exp Biol Med 157, 612–615 (2014). https://doi.org/10.1007/s10517-014-2627-5

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-014-2627-5

Key Words

Search

Navigation