Abstract
The aim of the present study was to determine the chemical composition of Sambucus ebulus and Actinidia deliciosa ethanolic extracts as well as their in vitro antifungal activity on the mycelial growth of the water mold, Saprolegnia parasitica. The preliminary minimum inhibition concentration (MIC) and minimum lethal concentration (MLC) were determined by the HeMP method and finally, concentrations of each extract ranging from 1 to 10% were prepared by an agar dilution method to assess the in vitro antifungal activity, quantitatively. Both herbal extracts inhibited growth of Saprolegnia hyphae in vitro. Complete in vitro growth inhibition was found at a concentration of ≥5% for S. ebulus, whereas it was not observed even at 10% concentration of A. deliciosa. Based on gas chromatography coupled with mass spectrometry (GC/MS) analysis, the main constituents of S. ebulus include monophthalate (66.17%), fatty acids (26.47%), phytol (4.25%), and acetic acid (2.11%). Using colorimetric assays, A. deliciosa contained phenolic contents at 162 mg gallic acid (GAE)/g DW and flavonoid contents at 2.31 mg quercetin (QE)/g DW. In conclusion, the results of this study indicated that S. ebulus and A. deliciosa showed some antifungal activities against S. parasitica with formerly exhibiting stronger activity (p < 0.05).
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The data that support the findings of this study are available on request from the corresponding author.
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This work was supported by the research council of the University of Tehran, Tehran, Iran.
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Experimental design: SM, MG, ARKh. Performing the experiments: SM and MG. data curation: SM, MG, and ARKh. Writing the paper: SM, MG, and ARKh.
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Mirmazloomi, S., Ghiasi, M. & Khosravi, A.R. Chemical composition and in vitro antifungal activity of Sambucus ebulus and Actinidia deliciosa on the fish pathogenic fungus, Saprolegnia parasitica. Aquacult Int 30, 1037–1046 (2022). https://doi.org/10.1007/s10499-022-00835-5
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DOI: https://doi.org/10.1007/s10499-022-00835-5