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PpV, acting via the JNK pathway, represses apoptosis during normal development of Drosophila wing

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Abstract

Apoptosis is one of the main fundamental biological processes required for development of multicellular organisms. Inappropriate regulation of apoptosis can lead to severe developmental abnormalities and diseases. Therefore, the control of apoptosis, not only for its activation but also for its inhibition, is critically important during development. In contrast to the extensive studies of apoptosis induction, its inhibitory mechanisms that are even more vital in certain populations of cells actually are very far from being well understood. Here we report an inhibitory role of protein phosphatase V (PpV), a serine/threonine protein phosphatase, in controlling the apoptosis during Drosophila wing development. We observed that inhibition of ppv by RNAi in wing imaginal discs induced ectopic cell death and caspase activation, thus, resulted in a defective adult wing. Moreover, knocking-down ppv triggered the activation of c-Jun N-terminal kinase (JNK) signal, an evolutionarily conserved intracellular signaling that has been implicated to modulate the apoptotic machinery in many biological and experimental systems. Disrupting the JNK signal transduction was adequate to suppress the ppv effects for wing development. Together, we provided the evidence to demonstrate that ppv is required for normal wing development in maintaining the silence of apoptotic signal possibly through JNK pathway.

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Acknowledgements

We thank the Bloomington Drosophila Stock Center, the Fly Stocks of National Institute of Genetics, the Vienna Drosophila RNAi Center, and the Tsinghua Stock Center, Shanghai Core Facility of Drosophila Resource and Technology CAS for fly stocks. This work was supported by the Fundamental Research Funds for the Central Universities (201612010 to Y. Su, 201762003 and 201562029), National Natural Science Foundation of China (31701274 to Y. Su), and China Postdoctoral Science Foundation (2017M612349 to Y. Su).

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  1. Institute of Evolution & Marine Biodiversity, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China

    Chunli Chi, Liguo Wang, Wenwen Lan & Ying Su

  2. Cardiovascular Research Center, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA, 02129, USA

    Long Zhao

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Chi, C., Wang, L., Lan, W. et al. PpV, acting via the JNK pathway, represses apoptosis during normal development of Drosophila wing. Apoptosis 23, 554–562 (2018). https://doi.org/10.1007/s10495-018-1479-2

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