Abstract
The current species of Halosegnis and Salella within the class Halobacteria are closely related based on phylogenetic, phylogenomic, and comparative genomic analyses. The Halosegnis species showed 99.8–100.0% 16S rRNA and 96.6–99.6% rpoB′ gene similarities to the Salella species, respectively. Phylogenetic and phylogenomic analyses showed that Salella cibi CBA1133T, the sole species of Salella, formed a single tight cluster with Halosegnis longus F12-1T, then with Halosegnis rubeus F17-44T. The average nucleotide identity (ANI), digital DNA–DNA hybridization (dDDH), and average amino acid identity (AAI) values between Salella cibi CBA1133T and Halosegnis longus F12-1T were 99.2, 94.2, and 98.6%, respectively, much higher than the thresholds for species demarcation. This genome-based classification revealed that the genus Salella should be merged with Halosegnis, and Salella cibi should be a later heterotypic synonym of Halosegnis longus. Halophilic archaeal strains DT72T, DT80T, DT85T, and DT116T, isolated from the saline soil of a tidal flat in China, were subjected to polyphasic taxonomic characterization. The phenotypic, chemotaxonomic, phylogenetic, and phylogenomic features indicated that strains DT72T (= CGMCC 1.18925T = JCM 35418T), DT80T (= CGMCC 1.18926T = JCM 35419T), DT85T (= CGMCC 1.19049T = JCM 35605T), and DT116T (= CGMCC 1.19045T = JCM 35606T) represent four novel species of the genera Halorussus, Halosegnis and Haloglomus, respectively, for which the names, Halorussus caseinilyticus sp. nov., Halorussus lipolyticus sp. nov., Halosegnis marinus sp. nov., and Haloglomus litoreum sp. nov., are proposed.
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The sequences determined in this study were deposited and available in the NCBI GenBank.
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Acknowledgements
We are grateful to the graduate student (Yu-Jie Xin, Master’s degree) for her primary contribution to this study.
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This work was financially supported by the National Natural Science Foundation of China (No. 32070003) and the National Science and Technology Fundamental Resources Investigation Program of China (No. 2021FY100900).
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YH, XM, ST, X-XL, MC performed the experiments and YH drafted the manuscript. JH and H-LC designed and supervised the study. JH and H-LC revised the manuscript. All authors of this article approved the submitted version.
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Hu, Y., Ma, X., Tan, S. et al. Genome-based classification of genera Halosegnis and Salella, and description of four novel halophilic archaea isolated from a tidal flat. Antonie van Leeuwenhoek 117, 51 (2024). https://doi.org/10.1007/s10482-024-01952-2
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DOI: https://doi.org/10.1007/s10482-024-01952-2