Abstract
The two novel bacterial strains, designated as VTT and ML, were isolated from roots of cinquefoil (Potentilla sp.) and leaves of meadow-grass (Poa sp.) on the flooded bank of lake, respectively. These isolates were Gram-negative, non-spore-forming, non-motile, rod-shaped cells, utilized methanol, methylamine, and polycarbon compounds as carbon and energy sources. In the whole-cell fatty acid pattern of strains prevailed C18:1ω7c and C19:0cyc. Based on the phylogenetic analysis of 16S rRNA gene sequences, strains VTT and ML were closely related to the representatives of the genus Ancylobacter (98.3–98.5%). The assembled genome of strain VTT has a total length of 4.22 Mbp, and a G + C content is 67.3%. The average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA–DNA hybridization (dDDH) values between strain VTT and closely related type strains of genus Ancylobacter were 78.0–80.6%, 73.8–78.3% and 22.1–24.0%, respectively, that clearly lower than proposed thresholds for species. On the basis of the phylogenetic, phenotypic, and chemotaxonomic analysis, isolates VTT and ML represent a novel species of the genus Ancylobacter, for which the name Ancylobacter radicis sp. nov. is proposed. The type strain is VTT (= VKM B-3255T = CCUG 72400T). In addition, novel strains were able to dissolve insoluble phosphates, to produce siderophores and plant hormones (auxin biosynthesis). According to genome analysis genes involved in the biosynthesis of siderophores, polyhydroxybutyrate, exopolysaccharides and phosphorus metabolism, as well as the genes involved in the assimilation of C1-compounds (natural products of plant metabolism) were found in the genome of type strain VTT.
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Abbreviations
- ANI:
-
Average nucleotide identity
- AAI:
-
Average amino acid identity
- dDDH:
-
Digital DNA–DNA hybridization
- PHB:
-
Polyhydroxybutyrate
- EPS:
-
Exopolysaccharides
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Funding
This work was supported by the Ministry of Science and Higher Education of the Russian Federation (Grant Number 075-15-2021-1051). Fatty acids analysis was performed by D.S.K. in Gubkin University and was supported by Russian Science Foundation (Grant Number 22-73-10224).
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N.V.A., experimental procedures, bioinformatic analysis, writing and approving the manuscript. A.A.B., experimental procedures, writing and approving the manuscript. E.N.K., sampling, experimental procedures, writing and approving the manuscript. S.V.T., experimental procedures, bioinformatic analysis, writing and approving the manuscript. D.S.K., experimental procedures, writing and approving the manuscript. A.V.M., experimental procedures, approving the manuscript. N.V.D., conceived the work, supervising experimental work, writing and approving the manuscript.
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Repositores: The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains VTT and ML are MT982345 and MW881039, respectively. The draft genome of the type strain VTT (= VKM B-3255T) has been deposited in NCBI GenBank under the accession number JAHCQH000000000.
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Agafonova, N.V., Belova, A.A., Kaparullina, E.N. et al. Ancylobacter radicis sp. nov., a novel aerobic methylotrophic bacteria associated with plants. Antonie van Leeuwenhoek 116, 855–866 (2023). https://doi.org/10.1007/s10482-023-01850-z
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DOI: https://doi.org/10.1007/s10482-023-01850-z