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Halobacillus naozhouensis sp. nov., a moderately halophilic bacterium isolated from a sea anemone

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Abstract

A moderately halophilic, Gram-positive, catalase- and oxidase-positive, rod-shaped, aerobic bacterium, designated strain JSM 071068T, was isolated from a sea anemone (Anthopleura xanthogrammica) collected from the Naozhou Island on the Leizhou Bay in the South China Sea. Cells were motile by means of peritrichous flagella and formed ellipsoidal endospores lying in subterminal swollen sporangia. Strain JSM 071068T was able to grow with 1–20% (w/v) total salts (optimum, 6–9%), at pH values of 6.0–10.0 (optimum, pH 7.5) and a temperature range of 10–35°C (optimum, 25°C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant menaquinone was MK-7 and the major cellular fatty acids were anteiso-C15:0, anteiso-C17:0 and iso-C15:0. The genomic DNA G + C content was 42.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 071068T belonged to the genus Halobacillus. The 16S rRNA gene sequence similarities between strain JSM 071068T and the type strains of the recognized Halobacillus species ranged from 97.9% (with Halobacillus alkaliphilus) to 95.3% (with Halobacillus kuroshimensis). The levels of DNA–DNA relatedness between the new isolate and the type strains of H. alkaliphilus, Halobacillus campisalis, Halobacillus halophilus and Halobacillus seohaensis were 25.6, 22.1, 10.8 and 13.2%, respectively. The combination of phylogenetic analysis, DNA–DNA relatedness, phenotypic characteristics and chemotaxonomic data supported the view that strain JSM 071068T represents a new species of the genus Halobacillus, for which the name Halobacillus naozhouensis sp. nov. is proposed, with JSM 071068T (=DSM 21183T =KCTC 13234T) as the type strain.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (NSFC) (30860013, 30860002, 30660004), Jishou University (jsdxkyzz200811), the Ministry of Science and Technology, PR China (2006BAE01A01-9, 863 Program, no. 2007AA021306), Key Projects of International Cooperation (2007DFB31620), the Yunnan Provincial Sciences and Technology Department (2005PY01-1, 2006C0006 M) and Yunnan University (2008YB005). We are grateful to Mr. Huai-Dong Xiao and Mr. Ke Huang for their excellent technical assistance.

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Authors and Affiliations

  1. College of Biology and Environmental Sciences, College of Chemistry and Chemical Engineering, Jishou University, 416000, Jishou, People’s Republic of China

    Yi-Guang Chen, Zhi-Xiong Liu, You-Xiang Zhang & Entomack Borrathybay

  2. The Key Laboratory for Microbial Resources of the Ministry of Education, Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, 650091, Kunming, People’s Republic of China

    Yi-Guang Chen, Shu-Kun Tang, Wen-Jun Li & Xiao-Long Cui

  3. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, 100050, Beijing, People’s Republic of China

    Yu-Qin Zhang

  4. Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 510301, Guangzhou, People’s Republic of China

    Wen-Jun Li

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  1. Yi-Guang Chen
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Correspondence to Yi-Guang Chen.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 071068T is EU925615.

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Chen, YG., Liu, ZX., Zhang, YQ. et al. Halobacillus naozhouensis sp. nov., a moderately halophilic bacterium isolated from a sea anemone. Antonie van Leeuwenhoek 96, 99–107 (2009). https://doi.org/10.1007/s10482-009-9340-9

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