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High mycobacterial diversity in recreational lakes

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Abstract

Although nontuberculous mycobacteria (NTM) are natural inhabitants of freshwater ecosystems, few studies have focused on their distribution in these habitats. Thus, the knowledge about the abundance as well as the composition of NTM remains limited and patchy in these environments. In this context, a prospective study was performed to identify favourable habitats for mycobacteria in two recreational lakes. Mycobacterial density and diversity were measured using quantitative real-time PCR and the MiSeq Illumina platform. For both lakes, five compartments were investigated, i.e. water column, air–water interface, sediment, epilithon and epiphyton biofilms. Nontuberculous mycobacteria were detected in all compartments in large densities and displayed a remarkable diversity. NTM were dominated by fast-growing species. Lakes and compartments appeared to shape mycobacteria assemblage composition as well as their densities. In both lakes, some OTUs assigned to the species level were identified as related to known opportunistic pathogens.

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Acknowledgments

This work was supported by the French National Research Agency through the Pulse (peri-urban lakes, society and environment) research project [ANR-10-CEPL-010] and by Eau de Paris. The authors are grateful to the Créteil leisure base and the Green Space and Environment Department of Paris for their authorization to collect samples in both lakes and for lending their boats. We also thank Fabien Joux for showing us how to use and manufacture the metal screen, Sylvain Huon, Cécile Bernard and Brigitte Vinçon-Leite for respectively lending us the corer, the fluoroprobe and the submersive data logger. Finally, we warmly thank Gérard Lacroix for sediment C/N ratio analysis.

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  1. Laboratoire Eau Environnement et Systèmes Urbains (LEESU UMR MA 102), Faculté des Sciences et Technologie, Université Paris-Est, AgroParisTech, 61 avenue du Général de Gaulle, 94000, Créteil, France

    A. Roguet, C. Therial, M. Saad, L. Boudahmane & F. S. Lucas

  2. Eau de Paris, Direction Recherche et Développement Qualité de l’Eau (DRDQE), 33 avenue Jean Jaurès, 94200, Ivry-Sur-Seine, France

    L. Moulin

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  1. A. Roguet
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Roguet, A., Therial, C., Saad, M. et al. High mycobacterial diversity in recreational lakes. Antonie van Leeuwenhoek 109, 619–631 (2016). https://doi.org/10.1007/s10482-016-0665-x

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