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Attachment and Biofilm Formation of Mycobacterium marinum on a Hydrophobic Surface at the Air Interface

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Abstract

Properties of attachment of Mycobacterium marinum to hydrophobic surfaces and subsequent␣biofilm formation were investigated. Binding of M. marinum to polypropylene occured under aerobic and anaerobic/microaerophilic conditions. However, aerobic conditions were necessary for biofilms to persist. Highly non-polar organic solvents were found to efficiently remove attached bacteria from the polypropylene surface, indicating strong hydrophobic interactions between the M. marinum cell wall and the surface. Increased capsular material, occurring during stationary phase, correlated with a decrease in attachment of cells to polypropylene. A protein of approximately 40 kDa appears to be present in increased amounts during the stationary phase. The protein has been identified by LC MS/MS analysis as alanine dehydrogenase.

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Acknowledgments

We are grateful to Dr. Robert Cole from The Johns Hopkins NHLBI Proteomic Center for his assistance in proteomic analysis, and to Dr. Sandra Honda of University of Maryland Biotechnology Institute for her help in revising this manuscript.

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Authors and Affiliations

  1. Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, MD, USA

    Mohammad R. Alavi, Hem D. Shukla & Manoucher Shahamat

  2. National Aquarium in Baltimore, Baltimore, MD, USA

    Brent Whitaker & Jill Arnold

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  1. Mohammad R. Alavi
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  2. Hem D. Shukla
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Correspondence to Mohammad R. Alavi.

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Alavi, M.R., Shukla, H.D., Whitaker, B. et al. Attachment and Biofilm Formation of Mycobacterium marinum on a Hydrophobic Surface at the Air Interface. World J Microbiol Biotechnol 23, 93–101 (2007). https://doi.org/10.1007/s11274-006-9198-1

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  • DOI: https://doi.org/10.1007/s11274-006-9198-1

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