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Dactylosporangium cerinum sp. nov., a novel actinobacterium isolated from the rhizosphere of Pinus koraiensis Sieb. et Zucc.

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Abstract

A novel actinobacterium, designated strain NEAU-TPG4T was isolated from rhizosphere soil of Pinus koraiensis Sieb. et Zucc. collected from Luobei, Heilongjiang Province, north China, and characterized using a polyphasic approach. Morphological and chemotaxonomic properties of strain NEAU-TPG4T were consistent with the description of the genus Dactylosporangium. The strain formed finger-shaped sporangia on short sporangiophores that emerged directly from substrate hyphae. The cell-wall peptidoglycan consisted of meso- and 3-hydroxy-diaminopimelic acids; arabinose, xylose and glucose were found as whole-cell sugars. The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones were identified as MK-9(H8) and MK-9(H6). The predominant cellular fatty acids were identified as iso-C16:0, anteiso-C15:0, anteiso-C17:0 and C18:0. Phylogenetic analysis using 16S rRNA gene sequences also indicated that the strain should be classified in the genus Dactylosporangium and showed that the closest relatives were Dactylosporangium maewongense Japan Collection of Microorganism (JCM) 15933T (99.6 %), Dactylosporangium siamense NBRC 106093T (99.6 %), Dactylosporangium aurantiacum JCM 3083T (99.5 %) and Dactylosporangium luteum JCM 17685T (99.5 %). However, the low level of DNA–DNA relatedness and several cultural and physiological characteristics allowed the strain to be distinguished from its closest relatives. Thus, it is proposed that strain NEAU-TPG4T represents a novel Dactylosporangium species. Dactylosporangium cerinum sp. nov. The type strain of D. cerinum is NEAU-TPG4T (=CGMCC 4.7152T = DSM 46712T).

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Acknowledgments

This work was supported in part by Grant from the Science and Technology Research Project of Heilongjiang Provincial Educational Commission (No. 12541001).

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    Authors and Affiliations

    1. Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

      Chongxi Liu, Xuejiao Guan, Pinjiao Jin, Jinmeng Li, Yao Li, Ying Zhou, Xiangjing Wang & Wensheng Xiang

    2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

      Lianjie Li, Yue Shen & Wensheng Xiang

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    1. Chongxi Liu
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    Correspondence to Xiangjing Wang or Wensheng Xiang.

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    Chongxi Liu and Xuejiao Guan contributed equally to this work.

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    Liu, C., Guan, X., Jin, P. et al. Dactylosporangium cerinum sp. nov., a novel actinobacterium isolated from the rhizosphere of Pinus koraiensis Sieb. et Zucc.. Antonie van Leeuwenhoek 108, 191–199 (2015). https://doi.org/10.1007/s10482-015-0478-3

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