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Planomonospora algeriensis sp. nov., an actinobacterium isolated from a Saharan soil of Algeria

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Abstract

A filamentous actinobacterium, designated strain PM3T, was isolated from a Saharan soil sample collected from Béni-Abbès, Béchar (South-West Algeria). A polyphasic taxonomic study was carried out to establish the status of strain PM3T. The isolate was found to have morphological and chemotaxonomical properties associated with members of the genus Planomonospora. The new isolated microorganism developed cylindrical sporangia arranged in double parallel rows on aerial mycelium, each one containing a motile single sporangiospore. The cell wall of the strain was found to contain meso-diaminopimelic acid. Whole-cell hydrolysates were found to contain madurose, glucose, mannose and ribose. The predominant menaquinone was identified as MK-9(H2) (69.6%). The polar lipids detected were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, phosphatidylhydroxyethanolamine and glucosamine-containing lipids. The major fatty acids were found to be C17:1ω9c (38.6%) and C17:0 (24.2%). Results of 16S rRNA gene sequence comparison revealed that strain PM3T shared a high degree of 16S rRNA gene sequence similarity with Planomonospora sphaerica DSM 44632T (99.3%), Planomonospora parontospora subsp. parontospora DSM 43177T (99.2%) and P. parontospora subsp. antibiotica DSM 43869T (99.0%). DNA–DNA hybridization values between strain PM3T and the type strains of the closely related species were between 58.4 and 70.1%. The combination of phylogenetic analysis, DNA–DNA relatedness data, phenotypic characteristics and chemotaxonomic data support the conclusion that strain PM3T represents a novel species of the genus Planomonospora, for which the name Planomonospora algeriensis sp. nov. is proposed. The type strain is PM3T (=DSM 46752T = CECT 9047T).

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Acknowledgements

We would like to gratefully acknowledge the technical assistance of Bettina Sträubler (DSMZ).

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Authors and Affiliations

  1. Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria

    Fawzia Chaabane Chaouch, Noureddine Bouras, Salim Mokrane, Khaoula Bouznada, Abdelghani Zitouni & Nasserdine Sabaou

  2. Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaïa, BP 455, 47000, Ghardaïa, Algeria

    Noureddine Bouras

  3. Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Braunschweig, Germany

    Gabriele Pötter & Cathrin Spröer

  4. School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK

    Hans-Peter Klenk

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  1. Fawzia Chaabane Chaouch
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  2. Noureddine Bouras
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Correspondence to Hans-Peter Klenk or Nasserdine Sabaou.

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This article does not contain any studies with human participants and/or animals performed by any of the authors. The formal consent is not required in this study.

Additional information

The GenBank Accession Number for the 16S rRNA gene sequence of strain PM3T is KX029461.2.

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Chaabane Chaouch, F., Bouras, N., Mokrane, S. et al. Planomonospora algeriensis sp. nov., an actinobacterium isolated from a Saharan soil of Algeria. Antonie van Leeuwenhoek 110, 245–252 (2017). https://doi.org/10.1007/s10482-016-0795-1

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