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Streptomyces gulbargensis sp. nov., isolated from soil in Karnataka, India

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Abstract

During the course of screening for industrially important microorganisms, an alkalitolerant and thermotolerant actinomycete, strain DAS 131T, was isolated from a soil sample collected from the Gulbarga region, Karnataka province, India. The strain was characterized by a polyphasic approach that showed that it belonged to the genus Streptomyces. Growth was observed over a wide pH range (pH 6–12) and at 45°C. The 16S rRNA gene sequence of strain DAS 131T was deposited in the GenBank database under the accession number DQ317411. 16S rRNA gene sequence analysis revealed that strain DAS 131Twas most closely related to Streptomyces venezuelae ISP 5230T (AY999739) with a sequence similarity of 99.5% (8 nucleotide differences out of 1,477). Despite this very high sequence similarity, strain DAS 131T was phenetically distinct from S. venezuelae. The DNA relatedness between these strains was 54%, indicating that strain DAS 131T is a distinct genomic species. On the basis of phenetic and genetic analyses, strain DAS 131T is classified as a new species in the genus Streptomyces, for which we propose the name Streptomyces gulbargensis sp. nov.

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Authors and Affiliations

  1. The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650 091, P.R. China

    Syed G. Dastager, Wen-Jun Li, Shu-Kun Tang, Xin-Peng Tian & Xiao-Yang Zhi

  2. Department of Studies & Research in Microbiology, Gulbarga University, Gulbarga, 585 106, Karnataka, India

    Syed G. Dastager, Dayanand Agasar & Mudgulkar B. Sulochana

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  1. Syed G. Dastager
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  2. Wen-Jun Li
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  3. Dayanand Agasar
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  4. Mudgulkar B. Sulochana
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  5. Shu-Kun Tang
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  6. Xin-Peng Tian
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  7. Xiao-Yang Zhi
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Correspondence to Dayanand Agasar.

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G. Dastager, S., Li, WJ., Agasar, D. et al. Streptomyces gulbargensis sp. nov., isolated from soil in Karnataka, India. Antonie van Leeuwenhoek 91, 99–104 (2007). https://doi.org/10.1007/s10482-006-9099-1

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