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In Vitro Models for Studying Transport Across Epithelial Tissue Barriers

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Abstract

Epithelial barriers are the body’s natural defense system to regulating passage from one domain to another. In our efforts to understand what can and cannot cross these barriers, models have emerged as a reductionist approach to rigorously study and investigate this question. In particular, in vitro tissue models have become prominent as there is an increased exploration of understanding biological molecular transport. Herein, we introduce the pertinent physiology, then discuss recent studies and approaches for building models of five epithelial tissues: skin, the gastrointestinal tract, the lungs, the blood–brain barrier, and the placenta. In particular, we evaluated literature from the past 5 years utilizing a tissue model to evaluate molecular transport. We then compare physiology of these tissues and discuss similarities in approaches, across tissues, to validate these models. We conclude with a summary of the approaches of growing interest across multiple tissues and an outlook on future steps to improve these models.

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(Adapted from Klein et al., Part Fibre Toxicol, 2013).

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(Adapted from Prabhakarpandian, et al., Lab Chip, 2013).

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Acknowledgments

This work was supported by a seed grant from the Sheikh Zayed Institute for Pediatric Surgical Innovation at Children’s National Medical Center and the A. James Clark School of Engineering at the University of Maryland. J.N. acknowledges and thanks support from the Fulbright Scholars Program. The content is solely the responsibility of the authors and does not necessarily represent the official views of these funding sources.

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None of the authors have competing interests with the work presented herein.

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Authors and Affiliations

  1. Fischell Department of Bioengineering, University of Maryland, College Park, 4102A Clark Hall, 8278 Paint Branch Drive, College Park, MD, 20742, USA

    Navein Arumugasaamy, Javier Navarro & John P. Fisher

  2. Center for Engineering Complex Tissues, University of Maryland, College Park, College Park, MD, 20742, USA

    Navein Arumugasaamy, Javier Navarro & John P. Fisher

  3. Sheikh Zayed Institute for Pediatric Surgical Innovation, Children’s National Health System, Washington, D.C, 20010, USA

    Navein Arumugasaamy, Peter C. W. Kim & John P. Fisher

  4. Department of Biomedical Engineering, University of California, Davis, Davis, CA, 95616, USA

    J. Kent Leach

  5. School of Medicine and Health Sciences, The George Washington University, Washington, DC, 20052, USA

    Peter C. W. Kim

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  1. Navein Arumugasaamy
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Correspondence to John P. Fisher.

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Associate Editor Smadar Cohen oversaw the review of this article.

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Arumugasaamy, N., Navarro, J., Kent Leach, J. et al. In Vitro Models for Studying Transport Across Epithelial Tissue Barriers. Ann Biomed Eng 47, 1–21 (2019). https://doi.org/10.1007/s10439-018-02124-w

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  • DOI: https://doi.org/10.1007/s10439-018-02124-w

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