Abstract
Irrigation with saline water can act as an alternate water resource and thus plays an important role in saving freshwater resources as well as promoting agriculture. Furthermore, salinity stress is considered one of the major abiotic stress factors, which strongly reduces crop productivity. In this context, the present work was conducted to examine the effect of exogenous salicylic acid (SA) application on salt stress tolerance of strawberry plants. For this purpose, strawberry plants (Fragaria vesca L.), three months old, were treated with three SA concentrations (0 mM, 0.25 mM and 0.5 mM), then subjected to 80 mM NaCl or not. After five weeks of treatment, growth responses, water status, photochemical efficiency and oxidative stress indicators were measured. The obtained results showed that irrigation with saline water negatively affected the growth parameters, the leaf water potential (LWP), the relative water content (RWC), the stomatal conductance (gs) and photochemical efficiency (Fv/Fm). While, the total protein content, the electrolyte leakage (EL), the malondialdehyde (MDA) and the hydrogen peroxide (H2O2) contents were increased in stressed plants compared to unstressed ones. Salt stress also leads to the activation of the antioxidant enzymes. However, the exogenous application of SA under salt stress conditions reduced the H2O2 accumulation, the electrolyte leakage and the MDA content. It has also improved the growth parameters, the LWP, the RWC, the gs, the Fv/Fm, the protein content and the antioxidant enzyme activities (POD, CAT and SOD) in the treated plants compared to those without SA application. Therefore, the beneficial effect of 0.25 mM SA on Fragaria vesca L. salinity tolerance may provide some practical basis for strawberry cultivation under saline conditions.
Zusammenfassung
Die Bewässerung mit salzhaltigem Wasser kann als alternative Wasserressource dienen und spielt somit eine wichtige Rolle bei der Einsparung von Süßwasserressourcen sowie bei der Förderung der Landwirtschaft. Darüber hinaus wird Salzstress als einer der wichtigsten abiotischen Stressfaktoren angesehen, die die Produktivität der Pflanzen stark reduzieren. In diesem Zusammenhang wurde die vorliegende Arbeit durchgeführt, um den Effekt einer exogenen Salicylsäure(SA)-Applikation auf die Toleranz gegenüber Salzstress bei Erdbeeren zu untersuchen. Zu diesem Zweck wurden drei Monate alte Erdbeerpflanzen (Fragaria vesca L.) mit drei SA-Konzentrationen (0 mM, 0.25 mM und 0.5 mM) behandelt und anschließend 0 oder 80 mM NaCl ausgesetzt. Nach fünf Wochen Behandlung wurden Wachstumsreaktionen, Wasserstatus, photochemische Effizienz und Indikatoren für oxidativen Stress gemessen. Die erhaltenen Ergebnisse zeigten, dass die Bewässerung mit Salzwasser die Wachstumsparameter, das Blattwasserpotenzial (LWP), den relativen Wassergehalt (RWC), die stomatäre Leitfähigkeit (gs) und die photochemische Effizienz (Fv/Fm) negativ beeinflusste. Der Gesamtproteingehalt, der Elektrolytverlust, der Malondialdehyd(MDA)-Gehalt und der H2O2-Gehalt in den Blättern der gestressten Pflanzen waren im Vergleich zu den nicht gestressten Pflanzen erhöht. Der Salzstress (80 mM NaCl) führte auch zur Aktivierung von antioxidativen Enzymen. Die exogene Applikation von SA unter Salzstressbedingungen reduzierte jedoch die H2O2-Akkumulation, den Elektrolytverlust und den MDA-Gehalt. Sie verbesserte auch die Wachstumsparameter, das Blattwasserpotenzial (LWP), den relativen Wassergehalt (RWC), die stomatäre Leitfähigkeit (gs) und die photochemische Effizienz (Fv/Fm), den Proteingehalt und die Aktivitäten der antioxidativen Enzyme (POD, CAT und SOD) in den behandelten Pflanzen im Vergleich zu Pflanzen ohne SA-Applikation. Der positive Effekt von 0,25 mM SA auf die Salinitätstoleranz von F. vesca könnte daher eine praktische Grundlage für den Erdbeeranbau unter salzhaltigen Bedingungen darstellen.
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References
Abdel-Lattif HM, Abbas MS, Taha MH (2019) Effect of salicylic acid on productivity and chemical constituents of some wheat (Triticum aestivum L.) varieties grown under saline conditions. J Anim Plant Sci 29:1054–1064
Ahanger MA, Aziz U, Alsahli AA et al (2020) Influence of exogenous salicylic acid and nitric oxide on growth, photosynthesis, and ascorbate-glutathione cycle in salt stressed vigna angularis. Biomolecules. https://doi.org/10.3390/biom10010042
Ahmad P, Alyemeni MN, Ahanger MA et al (2018) Salicylic acid (SA) induced alterations in growth, biochemical attributes and antioxidant enzyme activity in faba bean (Vicia faba L.) seedlings under nacl toxicity. Russ J Plant Physiol 65:104–114. https://doi.org/10.1134/S1021443718010132
Alsahli A, Mohamed AK, Alaraidh I et al (2019) Salicylic acid alleviates salinity stress through the modulation of biochemical attributes and some key antioxidants in wheat seedlings. Pak J Bot 51:1551–1559. https://doi.org/10.30848/PJB2019-5(12)
Amirinejad AA, Sayyari M, Ghanbari F, Kordi S (2017) Salicylic acid improves salinityalkalinity tolerance in pepper (Capsicum annuum l.). Adv Hortic Sci 31:157–163. https://doi.org/10.13128/ahs-21954
Anaya F, Fghire R,Wahbi S, Loutfi K (2017) Antioxidant enzymes and physiological traits of Vicia faba L. as affected by salicylic acid under salt stress. J Mater Environ Sci 8:2549–2563
Anaya F, Fghire R, Wahbi S, Loutfi K (2018) Influence of salicylic acid on seed germination of Vicia faba L. under salt stress. J Saudi Soc Agric Sci 17(1):1–8. https://doi.org/10.1016/j.jssas.2015.10.002
Arbaoui M, Belkhodja M (2018) Metabolic response of tomato (Lycopersicon esculentum Mill.) under salt stress combined with hormones. Int J Agron Agri Res 12:37–45
Barrs H, Weatherley P (1962) A re-examination of the relative turgidity technique for estimating water deficits in leaves. Aust J Biol Sci 15:413. https://doi.org/10.1071/bi9620413
Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287. https://doi.org/10.1016/0003-2697(71)90370-8
Bose J, Rodrigo-Moreno A, Shabala S (2014) ROS homeostasis in halophytes in the context of salinity stress tolerance. J Exp Bot 65:1241–1257. https://doi.org/10.1093/jxb/ert430
Bradford MM (1976) A Rapid and sensitive Method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254. https://doi.org/10.1016/0003-2697(76)90527-3
Cai H, He M, Ma K et al (2015) Salicylic acid alleviates cold-induced photosynthesis inhibition and oxidative stress in Jasminum sambac. Turk J Biol 39:241–247. https://doi.org/10.3906/biy-1406-35
Dalcin JS, Nunes UR, Roso R et al (2019) Salicylic acid concentrations and its effects on the physiological quality of soybean seeds. J Agric Sci 11:271. https://doi.org/10.5539/jas.v11n17p271
El Moujabber M, Atallah T, Darwish T, Ndayra G (2006) Etude De La Tolérance De La Fraise (Fragaria Vivace) À La Salinité Au Liban. Leban Sci J 7:33–44
Elwan MWM, Elhamahmy MAM, Mohamed FH (2018) Physiological effect of potato genotypes and Salicylic acid on plantlets growth and microtuber production under salt stress. Hortscience J Suez Canal Univ 7:7–14. https://doi.org/10.21608/hjsc.2018.59092
FAO (2015) Status of the world’s soil resources, intergovernmental technical panel on soils
Faried HN, Ayyub CM, Amjad M et al (2017) Salicylic acid confers salt tolerance in potato plants by improving water relations, gaseous exchange, antioxidant activities and osmoregulation. J Sci Food Agric 97:1868–1875. https://doi.org/10.1002/jsfa.7989
Fghire R, Ali OI, Anaya F, Benlhabib O, Jacobsen SE, Wahbi S (2013) Protective antioxidant enzyme activities are affected by drought in Quinoa (Chenopodium quinoa Willd). J Biol Agric Healthc 3(4):62–68.
Fghire R, Anaya F, Ali OI, Benlhabib O, Ragab R, Wahbi S (2015) Physiological and photosynthetic response of quinoa to drought stress. Chil J Agric Res 75(2):174–183. https://doi.org/10.4067/S0718-58392015000200006
Fghire R, Anaya F, Issa OA, Wahbi S (2017) Physiological and growth response traits to water deficit as indicators of tolerance criteria between quinoa genotypes. J Mater Environ Sci 8:2084–2093
Ghassemi-Golezani K, Farhangi-Abriz S, Bandehagh A (2018) Salicylic acid and jasmonic acid alter physiological performance, assimilate mobilization and seed filling of soybean under salt stress. Acta Agric Slov 111:597–607. https://doi.org/10.14720/aas.2018.111.3.08
Gong Y, Toivonen PMA, Lau OL, Wiersma PA (2001) Antioxidant system level in “Braeburn” apple is related to its browning disorder. Bot Bull Acad Sin 42:259–264
Haydari M, Maresca V, Rigano D et al (2019) Salicylic acid and melatonin alleviate the effects of heat stress on essential oil composition and antioxidant enzyme activity in Mentha × Piperita and Mentha Arvensis L. Antioxidants 8:1–12. https://doi.org/10.3390/antiox8110547
Hodges DM, DeLong JM, Forney CF, Prange RK (1999) Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207:604–611. https://doi.org/10.1007/s004250050524
Hori K, Wada A, Shibuta T (1997) Changes in phenoloxidase activities of the galls on leaves of ulmus davidana formed by tetraneura fusiformis (Homoptera: Eriosomatidae). Appl Entomol Zool 32:365–371. https://doi.org/10.1303/aez.32.365
Issa Ali O, Fghire R, Anaya F et al (2019) Physiological and morphological responses of two Quinoa Cultivars (Chenopodium quinoa Willd.) to drought stress. Gesunde Pflanz 71:123–133. https://doi.org/10.1007/s10343-019-00460-y
Kaya C, Kirnak H, Higgs D, Saltali K (2002) Supplementary calcium enhances plant growth and fruit yield in strawberry cultivars grown at high (NaCL) salinity. Sci Hortic 93:65–74. https://doi.org/10.1016/S0304-4238(01)00313-2
Keutgen AJ, Pawelzik E (2009) Impacts of NaCl stress on plant growth and mineral nutrient assimilation in two cultivars of strawberry. Environ Exp Bot 65:170–176. https://doi.org/10.1016/j.envexpbot.2008.08.002
Kevork Sajyan T, Shaban N, Rizkallah J, Najib Sassine Y (2019) Performance of salt-stressed tomato crop as affected by Nano-Caco3, glycine betaine, Mkp fertilizer and aspirin application. J Agric For 65:19–27. https://doi.org/10.17707/agricultforest.65.1.02
Al Kharusi L, Al Yahyai R, Yaish MW (2019) Antioxidant response to salinity in salt-tolerant and salt-susceptible cultivars of date palm. Agric 9:1–17. https://doi.org/10.3390/agriculture9010008
Ko MJ, Jayaramaiah RH, Gupta R et al (2017) Evaluation of bioactive compounds in strawberry fruits by a targeted metabolomic approach. Korean J Hortic Sci Technol 35:805–819. https://doi.org/10.12972/kjhst.20170084
Kumar Singh A, Dwivedi P (2018) Modulation of salt stress induced responses in pea (Pisum sativum L.) through salicylic acid and trichoderma application. Int J Curr Microbiol Appl Sci 7:3173–3185. https://doi.org/10.20546/ijcmas.2018.704.360
Lutts S, Kinet JM, Bouharmont J (1996) NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Ann Bot 78:389–398. https://doi.org/10.1006/anbo.1996.0134
MAMDA (2017) Dossier fruits rouges, Agrumes la profession se mobilise. Agric du Maghreb 102:1–64. http://www.agrimaroc.ma/maroc-fraiseframboise-cerise/. Accessed 6 June 2019
Marengo JA, Torres RR, Alves LM (2017) Drought in Northeast Brazil—past, present, and future. Theor Appl Climatol 129:1189–1200. https://doi.org/10.1007/s00704-016-1840-8
MEMECEE (2011) Rapport de Diagnostique de l’Etat de l’Environnement au Maroc (Maroc: Ministère de l’Energie, des Mines, de l’Eau et de l’Environnement, Chargé de l’Eau et de l’Environnement Département de l’Environnement). (http://www.environnement.gov.ma/fr/9-non-categorise/122-rapportnational-sur-l-etat-de-l-environnement/le06/06/2020).
Mimouni H, Wasti S, Manaa A et al (2016) Does salicylic acid (SA) improve tolerance to salt stress in plants? A study of SA effects on tomato plant growth, water dynamics, photosynthesis, and biochemical parameters. Omi A J Integr Biol 20:180–190. https://doi.org/10.1089/omi.2015.0161
Miura K, Tada Y (2014) Regulation of water, salinity, and cold stress responses by salicylic acid. Front Plant Sci 5:1–12. https://doi.org/10.3389/fpls.2014.00004
Mohamed FHEMWM (2018) Physiological effect of potato genotypes and salicylic acid on Plantlets growth and microtuber production under salt stress. Hortsci J Suez Canal Univ 7:7–14. https://doi.org/10.21608/hjsc.2018.59092
Otálora G, Piñero MC, Collado-González J et al (2020) Exogenous salicylic acid modulates the response to combined salinity-temperature stress in pepper plants (Capsicum annuum l. var. tamarin). Plants 9:1–12. https://doi.org/10.3390/plants9121790
Pandey AK, Lal EP (2018) Effect of salicylicacid on morphological, biochemical and antioxidant parameters of mungbean (vignaradiata L.) under salt stress. Plant Arch 18:109–116
Polash MAS, Sakil MA, Hossain MA (2019) Plants responses and their physiological and biochemical defense mechanisms against salinity: a review. Trop Plant Res 6:250–274. https://doi.org/10.22271/tpr.2019.v6.i2.35
Rajeshwari V, Bhuvaneshwari V (2017) Salicylic acid induced salt stress tolerance in plants. Int J Plant Biol Res 5:1067
Riaz A, Rafique M, Aftab M et al (2019) Mitigation of salinity in chickpea by plant growth promoting rhizobacteria and salicylic acid. Eurasian J Soil Sci 8:221–228. https://doi.org/10.18393/ejss.560745
Ribeiro JES, Sousa LV, Silva TI et al (2020) Citrullus lanatus morphophysiological responses to the combination of salicylic acid and salinity stress. Rev Bras Cienc Agrar Braz J Agric Sci 15:1–13. https://doi.org/10.5039/agraria.v15i1a6638
Salingpa TW, Lal EP, Shukla PK et al (2018) Effect of foliar application of salicylic acid on growth, yield, physiological and biochemical characteristics of mung bean (Vigna radiata L.) under salt stress. ~ 1857. J Pharmacogn Phytochem 7:1857–1860
Sharma A, Sidhu GPS, Araniti F et al (2020) The role of salicylic acid in plants exposed to heavy metals. Molecules 25:1–22. https://doi.org/10.3390/molecules25030540
Silva T, Filho J, Gonçalves A et al (2018) Salicylic acid effect on Ocimum basilicum L. during growth in salt stress and its relationship between phytomass and gas exchang. J Exp Agric Int 22:1–10. https://doi.org/10.9734/jeai/2018/40901
Solanki Mital V, Trivedi Sandhya K, Kandoliya UK, Golakiya BA (2018) Effect of exogenous application of salicylic acid on antioxidative enzymes in black gram (Vigna mungo (L.) Hepper) irrigated with saline water. Int J Chem Stud 6:2107–2116
De Souza Lucena CY, Rocha dos Santos DJ, Santos da Silva PL, Dantas da Costa E, Lucena RL (2018) O reuso de águas residuais como meio de convivência com a seca no semiárido do Nordeste Brasileiro. Regne 4:1–17
Torun H (2019) Time-course analysis of salicylic acid effects on ROS regulation and antioxidant defense in roots of hulled and hulless barley under combined stress of drought, heat and salinity. Physiol Plant 165:169–182. https://doi.org/10.1111/ppl.12798
Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain-treated bean plants protective role of exogenous polyamines. Plant Sci 151:59–66. https://doi.org/10.1016/S0168-9452(99)00197-1
Wang W, Wang X, Huang M et al (2018) Hydrogen peroxide and abscisic acid mediate salicylic acid-induced freezing tolerance in wheat. Front Plant Sci 9:1–13. https://doi.org/10.3389/fpls.2018.01137
Yu LL, Liu Y, Zhu F et al (2020) The enhancement of salt stress tolerance by salicylic acid pretreatment in arabidopsis thaliana. Biol plant 64:150–158. https://doi.org/10.32615/bp.2019.151
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K. Lamnai, F. Anaya, R. Fghire, H. Zine, S. Wahbi and K. Loutfi declare that they have no competing interests.
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Lamnai, K., Anaya, F., Fghire, R. et al. Impact of Exogenous Application of Salicylic Acid on Growth, Water Status And Antioxidant Enzyme Activity of Strawberry Plants (Fragaria vesca L.) Under Salt Stress Conditions. Gesunde Pflanzen 73, 465–478 (2021). https://doi.org/10.1007/s10343-021-00567-1
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DOI: https://doi.org/10.1007/s10343-021-00567-1
Keywords
- Salt stress
- Salicylic acid
- Strawberry (Fragaria vesca L.)
- Lipid peroxidation
- Enzyme activities
- Hydrogen peroxide