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Ultrasonic Nebulization-Dispersive Solid-Phase-Monolithic Microcolumn Extraction Coupled with HPLC–MS/MS for Analyzing Sex Steroids in Lotion Samples

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Abstract

An ultrasonic nebulization-dispersive solid-phase-monolithic microcolumn extraction method coupled with high-performance liquid chromatography–mass spectrometry was developed and applied for the extraction and analysis of 15 sex steroids in lotion samples. In this method, the analytes were microextracted from a liquid phase to a dispersive solid phase, and then, a monolithic microcolumn was prepared with these dispersive solid-phase particles using our device. In this way, a more efficient extraction method was developed. Several experimental parameters, including the extraction sorbent, amount of the adsorbent, extraction time, and extraction temperature, were optimized. Calibration curves for steroid concentrations ranging from 0.11 to 1461.00 ng mL−1 were obtained for analyzing the sex steroids. The recoveries in the determination of the sex steroids were between 53.19 and 96.99%, and the relative standard deviations ranged from 2.58 to 7.52%. These results indicated that the proposed method is a fast, sensitive, low-cost, and highly efficient extraction and enrichment technique for the analysis of nonvolatile analytes in liquid samples.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 81401744) and the Natural Science Basic Research Plan in Shaanxi Province of China (Program No. 2017JQ8013).

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  1. Department of Clinical Laboratory, Xijing Hospital, Fourth Military Medical University, Changle West Road 127, Xi’an, 710032, China

    Rui Zhang

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Zhang, R. Ultrasonic Nebulization-Dispersive Solid-Phase-Monolithic Microcolumn Extraction Coupled with HPLC–MS/MS for Analyzing Sex Steroids in Lotion Samples. Chromatographia 82, 1603–1615 (2019). https://doi.org/10.1007/s10337-019-03797-8

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  • DOI: https://doi.org/10.1007/s10337-019-03797-8

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