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Highly Sensitive Determination for Catecholamines Using Boronate Affinity Polymer Monolith Microextraction with In-Situ Derivatization and HPLC Fluorescence Detection

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Abstract

Catecholamines such as dopamine, norepinephrine and epinephrine are important neurotransmitters containing cis-diol moieties, and HPLC with fluorescence detection (HPLC-FD) has been successfully used as highly sensitive detection method for them. However, the complex matrix of biological samples is always a challenge. Boronate affinity microextraction is practical to purify and enrich cis-diol compounds. In this study, we used in-situ derivatization in boronate affinity polymer monolith microextraction (BA/PMME) to combine sample pretreatment and derivatization, then followed by HPLC-FD to develop a highly sensitive and selective determination method for catecholamines. Taking poly(3-acrylamidophenyl boronic acid-co-ethylene dimethacrylate) as BA/PMME material and TMBB-Su as labeling reagent, catecholamines could be derivatizated in polymer monolith in-situ together with the sample enrichment and purification. Comparing with commonly used solid phase extraction followed by derivatization, fewer operation steps are required and higher sensitivity was obtained with LODs as low as 0.06–0.2 nM.

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Funding

This study was funded by the National Nature Science Foundation of China (Grant Numbers 31670370 and 21777126) and the Natural Science Foundation of Hubei Province, China (2014CFA002).

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Author notes

  1. Wen-Le Fang and Li-Jun Xia contributed equally to this manuscript.

Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China

    Wen-Le Fang, Li-Jun Xia, Xiao Huang, Xiao-Feng Guo, Jun Ding, Hong Wang & Yu-Qi Feng

Authors
  1. Wen-Le Fang
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  2. Li-Jun Xia
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  3. Xiao Huang
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  4. Xiao-Feng Guo
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  5. Jun Ding
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  6. Hong Wang
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  7. Yu-Qi Feng
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Corresponding author

Correspondence to Hong Wang.

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The authors declare that they have no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. This article does not contain any studies with human participants performed by any of the authors.

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Fang, WL., Xia, LJ., Huang, X. et al. Highly Sensitive Determination for Catecholamines Using Boronate Affinity Polymer Monolith Microextraction with In-Situ Derivatization and HPLC Fluorescence Detection. Chromatographia 81, 1381–1389 (2018). https://doi.org/10.1007/s10337-018-3592-3

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  • DOI: https://doi.org/10.1007/s10337-018-3592-3

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