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Isolation of Aspartic Acid Using Novel Poly(2-hydroxyethyl methacrylate-N-methacryloyl-(l)-lysine) Cryogels

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Abstract

In this study, we report on the synthesis of novel poly(2-hydroxyethyl methacrylate-N-methacryloyl-(l)-lysine) [poly(HEMA-MAL)] cryogels for the isolation of aspartic acid. Cryogels were characterized using nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), N2-adsorption, swelling test, and elemental analysis techniques. The FTIR and elemental analysis results showed successful synthesis of poly(HEMA-MAL)-based polymeric structures. SEM images and N2 adsorption results indicated the three-dimensional morphology and large surface area of the cryogels. After the synthesis and characterization steps, adsorption performance of these novel cryogels towards l-aspartic acid was evaluated and adsorption capacity was found to be 265.7 ± 3 mg aspartic acid g−1 adsorbent. To show the applicability of our adsorbent design for the isolation of aspartic acid from real samples, adsorption and desorption experiments using lysozyme enzyme were also performed. Our results showed that the proposed cryogel design can be successfully employed for the isolation of amino acids from complex environments. It can be stated that poly(HEMA-MAL)-based cryogels are promising candidates for the isolation of aspartic acid and lysozyme owing to their enhanced performance and specificity, low cost, and high reusability.

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Acknowledgements

This work was supported by Hitit University Scientific Research Projects Coordination Department with the Grant Number of ODMYO19004.16.002.

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Authors and Affiliations

  1. Institute of Science and Technology, Hitit University, Çorum, Turkey

    Büşra Sarıca

  2. Alaca Avni Celik Vocational School, Hitit University, Çorum, Turkey

    Kazım Köse & Aytekin Uzunoğlu

  3. Osmancık Omer Derindere Vocational School, Hitit University, Çorum, Turkey

    Kadir Erol

  4. Department of Chemistry, Faculty of Science and Literature, Hitit University, Çorum, Turkey

    Dursun Ali Köse

Authors
  1. Büşra Sarıca
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  2. Kazım Köse
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  3. Aytekin Uzunoğlu
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  4. Kadir Erol
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  5. Dursun Ali Köse
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Correspondence to Aytekin Uzunoğlu.

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Sarıca, B., Köse, K., Uzunoğlu, A. et al. Isolation of Aspartic Acid Using Novel Poly(2-hydroxyethyl methacrylate-N-methacryloyl-(l)-lysine) Cryogels. Chromatographia 81, 127–137 (2018). https://doi.org/10.1007/s10337-017-3419-7

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  • DOI: https://doi.org/10.1007/s10337-017-3419-7

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