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Microfluidic study of effects of flow velocity and nutrient concentration on biofilm accumulation and adhesive strength in the flowing and no-flowing microchannels

  • Biotechnology Methods - Original Paper
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Journal of Industrial Microbiology & Biotechnology

Abstract

Biofilm accumulation in porous media can cause pore plugging and change many of the physical properties of porous media. Engineering bioplugging may have significant applications for many industrial processes, while improved knowledge on biofilm accumulation in porous media at porescale in general has broad relevance for a range of industries as well as environmental and water research. The experimental results by means of microscopic imaging over a T-shape microchannel clearly show that increase in fluid velocity could facilitate biofilm growth, but that above a velocity threshold, biofilm detachment and inhibition of biofilm formation due to high shear stress were observed. High nutrient concentration prompts the biofilm growth; however, the generated biofilm displays a weak adhesive strength. This paper provides an overview of biofilm development in a hydrodynamic environment for better prediction and modelling of bioplugging processes associated with porous systems in petroleum industry, hydrogeology and water purification.

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Acknowledgements

We wish to thank Edin Alagic, Rikke H. Ulvøen and Tove L. Eide for technical assistance. This work was supported by the Research Council of Norway and industry partner GOE-IP through the Project IMMENS No. 255426.

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Authors and Affiliations

  1. Centre for Integrated Petroleum Research (CIPR), Uni Research, Nygårdsgaten 112, 5008, Bergen, Norway

    Na Liu, Tormod Skauge, Beate Hovland, Bente Thorbjørnsen, Bartek Florczyk Vik & Gunhild Bødtker

  2. Department of Mathematics, Faculty of Mathematics and Natural Sciences, University of Bergen, Allégaten 41, P.O. Box 7803, 5020, Bergen, Norway

    David Landa-Marbán & Florin Adrian Radu

  3. Institute of Hydrochemistry, Technische Universität München, Marchioninistr. 17, 81377, Munich, Germany

    Thomas Baumann

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  1. Na Liu
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Liu, N., Skauge, T., Landa-Marbán, D. et al. Microfluidic study of effects of flow velocity and nutrient concentration on biofilm accumulation and adhesive strength in the flowing and no-flowing microchannels. J Ind Microbiol Biotechnol 46, 855–868 (2019). https://doi.org/10.1007/s10295-019-02161-x

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