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Streptomyces albulus yields ε-poly-l-lysine and other products from salt-contaminated glycerol waste

  • Bioenergy/Biofuels/Biochemicals - Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Actinomycetes are the most important microorganisms for the industrial production of secondary metabolites with antimicrobial and anticancer properties. However, they have not been implicated in biorefineries. Here, we study the ability of the ε-poly-l-lysine producing Streptomyces albulus BCRC 11814 to utilize biodiesel-derived crude glycerol. S. albulus was cultured in a mineral medium supplemented with up to 10% w/v sodium chloride or potassium chloride, and with crude glycerol as the sole carbohydrate source. Under these conditions, the strain produced 0.1 g ε-poly-l-lysine per 1 g of biomass. RNA sequencing revealed upregulation of the ectoine biosynthetic pathway of S. albulus, which provides proof of halotolerance. S. albulus has several silent secondary metabolite biosynthetic clusters predicted within the genome. Based on the results, we conclude that S. albulus BCRC 11814 is a halotolerant microorganism capable of utilizing biodiesel-derived crude glycerol better than other actinomycetes included in the present study. S. albulus has the potential to be established as microbial platform production host for a range of high-value biological products.

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Acknowledgements

We thank the National Research Foundation (NRF) and the Department of Science and Technology (DST) Biocatalysis Initiative for their financial assistance. The opinions expressed and conclusions arrived at are those of the authors and are not necessarily attributable to the NRF from whom Amanda Dodd received an Innovation Doctoral Scholarship (Grant ID 83765, National Research Foundation of South Africa). The authors have no conflict of interest to declare.

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Authors and Affiliations

  1. School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg, South Africa

    Amanda Dodd, Nerve Zhou & Karl Rumbold

  2. Biotechnology Platform, Agricultural Research Council, Onderstepoort, South Africa

    Dirk Swanevelder

  3. College of Agriculture and Environmental Sciences, University of South Africa, Pretoria, South Africa

    Dirk Swanevelder

  4. Molecular Sciences Institute, University of the Witwatersrand, Johannesburg, South Africa

    Dean Brady

  5. School of Biological Sciences, Queen’s University, Belfast, UK

    John E. Hallsworth

  6. Department of Biological Sciences and Biotechnology, Botswana International University of Science and Technology, Palapye, Botswana

    Nerve Zhou

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  1. Amanda Dodd
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Correspondence to Karl Rumbold.

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Dodd, A., Swanevelder, D., Zhou, N. et al. Streptomyces albulus yields ε-poly-l-lysine and other products from salt-contaminated glycerol waste. J Ind Microbiol Biotechnol 45, 1083–1090 (2018). https://doi.org/10.1007/s10295-018-2082-9

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