Abstract
Seed priming increases the vigor of seeds and seedlings through metabolic and biochemical processes occurring during controlled hydration, followed by dehydration. In the field, seeds are exposed to hydration-dehydration events in and on the soil after dispersal, as in seed priming. Nevertheless, seed priming has been sparsely tested on desiccation-sensitive seeds, which are vulnerable to climate change effects. We evaluated the effect of two priming methods on seeds from two tropical rainforest species: Cupania glabra and Cymbopetalum baillonii. For hydropriming, the seeds were fully hydrated and then dehydrated to three dehydration levels. For natural priming, the seeds were buried for 12 days in either closed forest or forest gap. Primed seeds were sown in 1% agar medium and placed in an environmental chamber. The growth of the seedlings from the highest germination priming treatments was evaluated for 1 year in the field. Our results showed that for C. glabra and C. baillonii, hydroprimed seeds varied in their germination response, depending on the degree of their dehydration. However, for C. baillonii, hydropriming seems to invigorate seeds, compared to non-imbibed seeds of the same dehydration level. Natural priming increased germination speed in both species without any difference between closed forest and forest gap. Moreover, seeds with natural priming had a higher final germination percentage than seeds with hydropriming. Seedlings from seeds with natural priming showed a higher growth rate than the controls in both species, whereas hydropriming produced a similar effect in C. glabra. Both priming methods could be used for restoration practices with the studied species, natural priming being a novel method. The ecological implications of priming in desiccation sensitive seeds are discussed in this study.
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Acknowledgements
We thank The Tropical Biological Station “Los Tuxtlas” (UNAM), Ismari Ramírez Lucho, Eladio Velasco, and María Esther Sánchez Coronado for their logistical and technical support. We also thank the Magallanes town in Tatahuicapan, Veracruz, and especially Juana Gutierres López, Simplicio Mateo González, Rubén Mateo Gutierres, and Iván Mateo Lorenzo for providing the area for experimental plot and for their assistance in the field. We thank Clara Leonor Tinoco Ojanguren, Cristina Martínez Garza, and Mark Olson for their important comments regarding this manuscript. We thank the editors and anonymous reviewers, whose comments improved this study. The author(s) disclose the receipt of the following financial support for the research, authorship, and/or publication of this article: This work was funded by the grants DGAPA-PAPIIT UNAM (201912, 205715) and CONACyT (221015).
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Becerra-Vázquez, Á.G., Coates, R., Sánchez-Nieto, S. et al. Effects of seed priming on germination and seedling growth of desiccation-sensitive seeds from Mexican tropical rainforest. J Plant Res 133, 855–872 (2020). https://doi.org/10.1007/s10265-020-01220-0
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DOI: https://doi.org/10.1007/s10265-020-01220-0