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A low-cost process for efficient hydrolysis of deoiled rice bran and ethanol production using an inhouse produced multi-enzyme preparation from Aspergillus niger P-19

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Abstract

The study deals with the management of deoiled rice bran by converting it into ethanol using a multi-enzyme preparation produced in-house on the same substrate. The thermo-acidic treatment with 1% H2SO4 released the highest reducing sugars amounting to 368 mg/g dry substrate (gds) upon enzymatic hydrolysis of pretreated mash, with 25% substrate loading, using multi-enzyme preparation from Aspergillus niger P-19. Steam pretreatment at 15 psi (pound-force per square inch) for 15 min yielding 356 mg/gds of reducing sugars was the best in terms of alcohol productivity (23 g/l) from the released sugars. The sugar yields were further improved to 468 mg/gds (117 g/l) of reducing sugars revealing 95.41% carbohydrate conversion efficiency and 370 mg/gds (92 g/l) of glucose using the statistical tool of response surface methodology for optimization of thermal pretreatment and enzymatic hydrolysis of deoiled rice bran. Upon fermentation with Saccharomyces cerevisiae, the sugars produced 37.63 g/l of ethanol with a yield of 0.41 g/g of sugars utilized. Thus, deoiled rice bran, a rich source of different carbohydrates, was effectively pretreated and enzymatically hydrolyzed to extract maximum sugars for conversion into bioethanol and the study thus holds the potential for validation at a pilot scale.

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Data availability

I, the corresponding author (Sanjeev Kumar Soni), declare on behalf of all the authors that as per the policy of the Journal, the data and material can be made available.

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Acknowledgements

The authors also acknowledge the assistance provided by the Central Instrumentation Laboratory at Sophisticated Analytical Instrumentation Facility (SAIF), Panjab University, Chandigarh, India, for FE-SEM, XRD and FTIR analysis.

Funding

The authors are highly thankful to the Department of Science and Technology DST), Ministry of Science and Technology, Government of India for the award of Research Fellowship and Contingency grant to Priya Chugh under the INSPIRE programme. The funding provided by the University Grants Commission (UGC), New Delhi under SAP is also acknowledged.

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Authors and Affiliations

  1. Department of Microbiology, Panjab University, Chandigarh, 160014, India

    Priya Chugh, Jaspreet Kaur, Apurav Sharma & Sanjeev Kumar Soni

  2. Department of Biotechnology, DAV College, Chandigarh, India

    Raman Soni

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  1. Priya Chugh
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  2. Jaspreet Kaur
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  3. Raman Soni
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Contributions

PC did experimental work, and wrote the original draft; JK did experimental work, and helped in writing the original draft; AS helped in experimental work, writing the original draft; RS supervised, and guided in writing, SKS helped in conceptualization, Supervision, and writing- editing.

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Correspondence to Sanjeev Kumar Soni.

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Chugh, P., Kaur, J., Soni, R. et al. A low-cost process for efficient hydrolysis of deoiled rice bran and ethanol production using an inhouse produced multi-enzyme preparation from Aspergillus niger P-19. J Mater Cycles Waste Manag 25, 359–375 (2023). https://doi.org/10.1007/s10163-022-01538-y

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