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Identification of microbial populations contributing to nitrification-associated nitrous oxide emission during cattle manure composting process with forced aeration

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Abstract

This study was conducted to clarify the fates of whole nitrogen cycle-associated microorganisms during laboratory-scale composting of cattle manure with continuous forced aeration, with the primary goal of identifying microbial populations that contributed greatly to high nitrous oxide (N2O) emissions triggered by nitrification. DNA microarray analysis targeting functional genes associated with the entire microbial nitrogen cycle was applied for the first time to investigate the fates of entire nitrogen cycle-associated microbial community during composting, and revealed increased diversity of nitrogen cycle-associated microorganisms, including ammonia-oxidizing populations, during active nitrification and N2O emission periods. Further analyses of the compositions of ammonia-oxidizing populations by DNA microarray, PCR-denaturing gradient gel electrophoresis and clone library methods revealed that Nitrosomonas europaea, Nitrosomonas eutropha, and their relatives were the dominant ammonia-oxidizing populations. Overall, the analyses of entire nitrogen cycle-associated microbial community and specific functional groups by a combination of different molecular biological methods in this study could confirm that N. europaea/eutropha and their relatives contributed greatly to high N2O emissions via active nitrification during cattle manure composting with forced aeration.

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Acknowledgements

This study was supported by the Core Research for Evolutionary Science and Technology (CREST) of the Japan Science and Technology Agency, and by a grant from the Kitasato University School of Allied Health Sciences (Grant-in-Aid for Research Project, No. 2014–1024).

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Authors and Affiliations

  1. Department of Health Science, School of Allied Health Sciences, Kitasato University, 1-15-1 Kitasato, Sagamihara-Minami, Kanagawa, 252-0373, Japan

    Daisuke Inoue & Kazuko Sawada

  2. Research and Education Faculty, Natural Sciences Cluster, Agricultural Unit, Kochi University, B200 Monobe, Nankoku, Kochi, 783-8502, Japan

    Hirofumi Tsutsui & Taku Fujiwara

  3. School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1, Katakura, Hachioji, Tokyo, 192-0982, Japan

    Hirofumi Tsutsui

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  1. Daisuke Inoue
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  2. Kazuko Sawada
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  3. Hirofumi Tsutsui
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  4. Taku Fujiwara
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Correspondence to Daisuke Inoue.

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Inoue, D., Sawada, K., Tsutsui, H. et al. Identification of microbial populations contributing to nitrification-associated nitrous oxide emission during cattle manure composting process with forced aeration. J Mater Cycles Waste Manag 20, 353–360 (2018). https://doi.org/10.1007/s10163-017-0588-2

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  • DOI: https://doi.org/10.1007/s10163-017-0588-2

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