Abstract
Genomic studies provide deeper insights into secondary metabolites produced by diverse bacterial communities, residing in various environmental niches. This study aims to understand the potential of a biosurfactant producing Bacillus sp. AM13, isolated from soil. An integrated approach of genomic and chemical analysis was employed to characterize the antibacterial lipopeptide produced by the strain AM13. Genome analysis revealed that strain AM13 harbors a nonribosomal peptide synthetase (NRPS) cluster; highly similar with known biosynthetic gene clusters from surfactin family: lichenysin (85 %) and surfactin (78 %). These findings were substantiated with supplementary experiments of oil displacement assay and surface tension measurements, confirming the biosurfactant production. Further investigation using LCMS approach exhibited similarity of the biomolecule with biosurfactants of the surfactin family. Our consolidated effort of functional genomics provided chemical as well as genetic leads for understanding the biochemical characteristics of the bioactive compound.
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Acknowledgments
Authors are thankful to Dr. Kiran Mahale (National Centre for Cell Science, Pune) and Dr. Sneha Sagarkar (Department of Biotechnology, Savitribai Phule University of Pune) for their valuable technical inputs. Authors are grateful to Mrs. Ashwini Rane (Department of Microbiology, Savitribai Phule University of Pune) for her assistance in conducting biosurfactant assay, and Datar Genetics Limited, Nashik, India for their support in Genome sequencing. We would also like to thank Dr. Ranadhir Chakraborty (North Bengal University) for providing the MDR isolates used in the study. This study was funded by Department of Biotechnology, Government of India, under the project of Microbial Culture Collection (Grant number: BT/PR10054/NDB/52/94/2007).
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An erratum to this article is available at http://dx.doi.org/10.1007/s10142-016-0528-6.
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Shaligram, S., Kumbhare, S.V., Dhotre, D.P. et al. Genomic and functional features of the biosurfactant producing Bacillus sp. AM13. Funct Integr Genomics 16, 557–566 (2016). https://doi.org/10.1007/s10142-016-0506-z
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DOI: https://doi.org/10.1007/s10142-016-0506-z