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Association Between Muscle Growth and Transcription of a Mutant MSTN Gene in Olive Flounder (Paralichthys olivaceus)

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Abstract

Myostatin (MSTN, also known as growth differentiation factor-8 (GDF-8)), a member of the transforming growth factor β (TGF-β) superfamily, functions as a negative regulator of skeletal muscle development and growth. However, it is also expressed in a wide range of tissues in fish and thus may have more diverse roles in this group than in mammals. In this study, we assessed the genome-wide transcriptional expression pattern associated with the CRISPR/Cas9-mutated MSTN gene in the olive flounder (Paralichthys olivaceus) in association with changes in cell proliferation and transportation processes. There were no differences in the hepatosomatic index, and the growth of male and female fish increased in the F1 progeny of the MSTN mutants. Furthermore, the histopathological analysis showed that myostatin editing resulted in a 41.24% increase in back muscle growth and 46.92% increase in belly muscle growth in male flounder compared with normal flounder, and a 16.01% increase in back muscle growth and 14.26% increase in belly muscle growth in female flounder compared with normal flounder. This study demonstrates that editing of the myostatin gene enhances muscle growth in olive flounder, with a notably more pronounced effect observed in males. Consequently, myostatin-edited male flounder could represent a valuable asset for the flounder aquaculture industry.

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Data Availability

The complete sequences generated in this study were deposited in the NCBI Sequence Read Archive under accession no. PRJNA1006540.

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Funding

This research was supported by the National Institute of Fisheries Science, Ministry of Oceans and Fisheries, Korea (grant number R2024031).

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Authors and Affiliations

  1. Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea

    Ju-Won Kim, Ja Young Cho, Young-Ok Kim & Hee Jeong Kong

  2. Genetics and Breeding Research Center, National Institute of Fisheries Science, Geoje, 53334, Republic of Korea

    Julan Kim

  3. Research and Development Center, Insilicogen Inc., Yongin-si, 16954, Republic of Korea

    Younhee Shin, Hyojung Son & Subramaniyam Sathiyamoorthy

  4. Department of Aquatic Life Medicine, Kunsan National University, Gunsan, 54150, Republic of Korea

    Bo-Seong Kim

  5. D.iF Inc., Yongin-si, 16954, Republic of Korea

    Byeong-chul Kang

Authors
  1. Ju-Won Kim
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  2. Julan Kim
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  4. Younhee Shin
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  8. Young-Ok Kim
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  9. Byeong-chul Kang
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Contributions

Conceptualization, J-w.K., Y.S., and B-c.K.; methodology, H.S., S.S., and B-s.K.; software, H.S.; validation, H.S., Y.S., and H.J.K.; formal analysis, H.S. and Y.S.; investigation, Y.S. and H.J.K.; resources, J.Y.C. and J.K.; data curation, H.S.; writing—original draft preparation, H.S., Y.S., and H.J.K.; and writing—review and editing, S.S., Y.S., and H.J.K.

Corresponding authors

Correspondence to Byeong-chul Kang or Hee Jeong Kong.

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Competing interests

The authors declare no competing interests.

Ethics Approval

Animal experiments were conducted in accordance with the Animal Protection Act of the Ministry of Agriculture, Food, and Rural Affairs, Republic of Korea, and approved by the Institutional Animal Care and Use Committee of the National Institute of Fisheries Science (2021-NIFS-IACUC-2).

Conflicts of Interest

Authors YS, HS, and SS were employed by Insilicogen Inc. and B-cK was employed by Di.F Inc. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest.

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Kim, JW., Kim, J., Cho, J.Y. et al. Association Between Muscle Growth and Transcription of a Mutant MSTN Gene in Olive Flounder (Paralichthys olivaceus). Mar Biotechnol (2024). https://doi.org/10.1007/s10126-024-10322-y

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