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First Report of Screening of Novel Angiotensin-I Converting Enzyme Inhibitory Peptides Derived from the Red Alga Acrochaetium sp.

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Abstract

ACE inhibitors generated from food proteins have recently become the most well-known subclass of bioactive peptides, and their bio-functionality can be a potential alternative to natural bioactive food components and synthetic drugs. The bioactivities of Acrochaetium sp., the red alga used in this investigation, have never been reported before. Screening of bioactive peptides from Acrochaetium sp. as ACE inhibitors were hydrolyzed with various proteolytic enzymes. Protein hydrolysates were fractionated separately using reversed phased (RP) and strong cation exchange (SCX) chromatography and identified as VGGSDLQAL (VL-9) using α-chymotrypsin. It comes from Phycoerythrin (PE), an abundant protein in a primarily red alga. The peptide VL-9 shows the ACE inhibitory activity with IC50 value 433.1 ± 1.08 µM. The inhibition pattern showed VL-9 as a non-competitive inhibitor. Molecular docking simulation proved that VL-9 was non-competitive inhibition due to the interaction peptide and ACE was not in the catalytic site. Moreover, VL-9 derived from Acrochaetium sp. is a natural bioactive peptide that is safer and available for food protein; also, the ACE inhibitory peptide derived from Acrochaetium sp. could be the one alternative resource to develop functional food for combating hypertension.

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Acknowledgements

We gratefully acknowledge the instrument support by Prof. J.L. Hsu from the Research Center of Active Natural Products Development, Department of Biological Science and Technology, and Precision Instrument Center of NPUST, Pingtung, Taiwan.

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Authors and Affiliations

  1. Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas Diponegoro, Semarang, 50275, Indonesia

    Seto Windarto

  2. Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan

    Seto Windarto & Jue-Liang Hsu

  3. Department of Aquaculture, College of Life Sciences, National Taiwan Ocean University, Keelung City, 20224, Taiwan

    Meng-Chou Lee

  4. Faculty of Fisheries and Marine Science, Universitas Brawijaya, Malang, 65145, Indonesia

    Seto Windarto & Happy Nursyam

  5. Research Center for Austronesian Medicine and Agriculture, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan

    Jue-Liang Hsu

  6. Research Center for Tropic Agriculture, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan

    Jue-Liang Hsu

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  1. Seto Windarto
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Windarto, S., Lee, MC., Nursyam, H. et al. First Report of Screening of Novel Angiotensin-I Converting Enzyme Inhibitory Peptides Derived from the Red Alga Acrochaetium sp.. Mar Biotechnol 24, 882–894 (2022). https://doi.org/10.1007/s10126-022-10152-w

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