Abstract
The study was conducted to assess the effects of nitrogen (N)-fixing purple nonsulfur bacteria (PNSB) Rhodopseudomonas palustris TLS06, VNW02, VNW64, and VNS89 on soil fertility, N uptake, essential oil (EO) content, growth, and yield of lemon balm. The experiment followed a completely randomized block design with 9 treatments and 3 replications. The treatments consisted of (i) applying 100% N as the recommended fertilizer rate (RFR), (ii) applying 85% N as RFR, (iii) applying 70% N as RFR, (iv) applying 55% N as RFR, (v) the treatment ii combined with N-PNSB, (vi) the treatment iii combined with N-PNSB, (vii) the treatment iv combined with N-PNSB, (viii) 0% as RFR combined with N-PNSB, and (ix) 0% N as RFR. The results showed that applying N-PNSB increased the plant height, and the number of primary branches in both seasons. In addition, the treatment without N fertilizer combined with N-PNSB increased stem leaf biomass by 41.2 and 50.3% in both seasons as compared with the treatment without neither N fertilizer nor N-PNSB. For soil properties, among treatments without N fertilizer, the treatment with N-PNSB increased concentrations of NH4+, soluble P, and exchangeable K+ by 41.3, 41.4, and 26.8%, respectively, as compared with the treatment without N-PNSB at the end of the second season. Applying 85% N as RFR combined with N-PNSB had a greater yield by 5.78–11.8% as compared with the treatment with 100% N as RFR, and a greater EO content by 23% as compared with the treatment with 85% N as RFR.
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The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
The authors sincerely appreciate the Purple Nonsulfur Bacteria Laboratory (Faculty of Crop Science, College of Agriculture, Can Tho University) provided equipment, chemicals, and materials for this study to be completed.
Funding
This work was supported by the Vietnam National University Ho Chi Minh City (VNU-HCM) [C2022-16–01].
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Le Thi My Thu: Conceptualization, Methodology, Data curation, Writing- Original draft preparation. Ly Ngoc Thanh Xuan: Investigation. Nguyen Huynh Minh Anh: Visualization, Investigation. Nguyen Duc Trong: Visualization, Investigation. Nguyen Thi Xuan Dao: Visualization, Investigation. Le Thanh Quang: Investigation, Writing- Reviewing and Editing. Le Thi Ngoc Tho: Validation. Ha Ngoc Thu: Investigation. Nguyen Tuan Anh: Investigation, Validation. Nguyen Thi Thuy Diem: Investigation, Validation, Writing- Reviewing and Editing. Nguyen Quoc Khuong: Conceptualization, Methodology, Writing- Reviewing and Editing. All authors read and approved the final manuscript.
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Thu, L.T.M., Xuan, L.N.T., Anh, N.H.M. et al. Improving nitrogen content in soil and lemon balm (Melissa officinalis L.) yield by purple nonsulfur bacteria Rhodopseudomonas palustris in two consecutive seasons. Int Microbiol (2024). https://doi.org/10.1007/s10123-024-00515-9
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DOI: https://doi.org/10.1007/s10123-024-00515-9