Abstract
Polycyclic aromatic hydrocarbons (PAHs) are considered substances of potential human health hazards because of their resistance to biodegradation and carcinogenic index. Chrysene is a PAH with a high molecular weight (HMW) that poses challenges for its elimination from the environment. However, bacterial degradation is an effective, environmentally friendly, and cost-effective solution. In our study, we isolated a potential chrysene-degrading bacteria from crude oil–contaminated seawater (Bizerte, Tunisia). Based on 16SrRNA analysis, the isolate S5 was identified as Achromobacter aegrifaciens. Furthermore, the results revealed that A. aegrifaciens S5 produced a biofilm on polystyrene at 20 °C and 30 °C, as well as at the air-liquid (A-L) interface. Moreover, this isolate was able to swim and produce biosurfactants with an emulsification activity (E24%) over 53%. Chrysene biodegradation by isolate S5 was clearly assessed by an increase in the total viable count. Confirmation was obtained via gas chromatography-mass spectrometry (GC-MS) analyses. A. aegrifaciens S5 could use chrysene as its sole carbon and energy source, exhibiting an 86% degradation of chrysene on day 7. In addition, the bacterial counts reached their highest level, over 25 × 1020 CFU/mL, under the conditions of pH 7.0, a temperature of 30 °C, and a rotary speed of 120 rpm. Based on our findings, A. aegrifaciens S5 can be a potential candidate for bioremediation in HMW-PAH-contaminated environments.
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Data availability
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
The authors are grateful to Mr. Nawali Hmida (Director of Laboratories of Tunis International Centre for Environmental Technologies (CITET, Tunis-Tunisia)) for his assistance and cooperation in the GC-MS analysis.
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This work was supported by the Tunisian Ministry of Higher Education, Scientific Research and Technology.
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Lazzem Assia, Chatti Abdelwaheb, and Elmay Alya. The first draft of the manuscript was written by Lazzem Assia and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Lazzem, A., Lekired, A., Ouzari, HI. et al. Isolation and characterization of a newly chrysene-degrading Achromobacter aegrifaciens. Int Microbiol (2023). https://doi.org/10.1007/s10123-023-00435-0
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DOI: https://doi.org/10.1007/s10123-023-00435-0